Western Blot (WB)
(Expression analysis of S100A8. Anti-S100A8 antibody (Clone: ABM4G30) was tested at 2 ug/ml on (1) human heart, (2) THP1 , (3) human lungs, (4) K562, and (5) HEK293 lysates.)
Mouse S100A8 Monoclonal Antibody | anti-S100A8 antibody
S100A8, human Monoclonal Antibody
Western Blot (WB)
(Expression analysis of S100A8. Anti-S100A8 antibody (Clone: ABM4G30) was tested at 2 ug/ml on (1) human heart, (2) THP1 , (3) human lungs, (4) K562, and (5) HEK293 lysates.)
Western Blot (WB)
(Expression analysis of S100A8. Anti-S100A8 antibody (Clone: ABM4G30) was tested at 2 ug/ml on (1) human heart, (2) THP1 , (3) human lungs, (4) K562, and (5) HEK293 lysates.)
NCBI and Uniprot Product Information
Uniprot Description
S100A8: S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH- oxidase by facilitating the enzyme complex assembly at the cell membrane, transfering arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF- kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Belongs to the S-100 family.
Cellular Component: extracellular space; cytoskeleton; membrane; cytoplasm; plasma membrane; extracellular region; nucleus
Molecular Function: arachidonic acid binding; antioxidant activity; RAGE receptor binding; zinc ion binding; metal ion binding; microtubule binding; calcium ion binding
Biological Process: caspase activation; neutrophil chemotaxis; immune system process; apoptosis; regulation of inflammatory response; autophagy; innate immune response; positive regulation of peptide secretion; inflammatory response; chemotaxis; leukocyte migration during inflammatory response; positive regulation of inflammatory response
Research Articles on S100A8
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Product Notes
The S100A8 s100a8 (Catalog #AAA668174) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The S100A8, human Monoclonal Antibody reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's S100A8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the S100A8 s100a8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S100A8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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