Rabbit anti-Human, Mouse RBM4 Polyclonal Antibody | anti-RBM4 antibody

RBM4 Antibody

Cat. Num. AAA7051320

• Gene Names: RBM4; LARK; RBM4A; ZCRB3A; ZCCHC21
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >95%, Protein G purified
• Synonyms: RBM4; Polyclonal Antibody; RBM4 Antibody; RNA-binding protein 4; Lark homolog; hLark; RNA-binding motif protein 4; RNA-binding motif protein 4a; RBM4A; anti-RBM4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 143

• Applicable Applications for anti-RBM4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1: 1000-1: 5000; IHC: 1: 20-1: 200
• Immunogen: Recombinant human RNA-binding protein 4 protein (121-205AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-98346
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in:Mouse brain tissueAll lanes: RBM4 antibody at 2.8ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 41,21,17,20 kDaObserved band size: 41 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human heart tissue using MBS7051320 at dilution of 1:100)

Related Product Information for anti-RBM4 antibody

RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3'-UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3'UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of AGO2-containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro.

NCBI and Uniprot Product Information

• NCBI GI #: 311771540
• NCBI GeneID: 5936
• NCBI Accession #: NP_001185772.1
• NCBI GenBank Nucleotide #: NM_001198843.1
• UniProt Accession #: Q9BWF3; O02916; Q4VC48; Q6P1P2; Q8WU85; B3KUN0; B4E1U0; E7EQS3
• Molecular Weight: 19,788 Da
• NCBI Official Full Name: RNA-binding protein 4 isoform 2
• NCBI Official Synonym Full Names: RNA binding motif protein 4
• NCBI Official Symbol: RBM4
• NCBI Official Synonym Symbols: LARK; RBM4A; ZCRB3A; ZCCHC21
• NCBI Protein Information: RNA-binding protein 4
• UniProt Protein Name: RNA-binding protein 4
• Protein Family: RNA-binding protein
• UniProt Gene Name: RBM4
• UniProt Synonym Gene Names: RBM4A; hLark
• UniProt Entry Name: RBM4_HUMAN

Uniprot Description

RBM4: RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3'-UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3'UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment (IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of EIF2C2- containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Nucleolus; RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: cytoplasm; nuclear speck; nucleolus; nucleoplasm; nucleus; stress granule

Molecular Function: miRNA binding; mRNA 3'-UTR binding; mRNA binding; protein binding; RNA binding

Biological Process: entrainment of circadian clock by photoperiod; miRNA-mediated gene silencing, negative regulation of translation; negative regulation of translation; negative regulation of translation in response to stress; negative regulation of translational initiation; positive regulation of muscle cell differentiation; regulation of alternative nuclear mRNA splicing, via spliceosome; regulation of nucleocytoplasmic transport; response to arsenic; RNA processing; stress-activated MAPK cascade