Western Blot (WB)
(All lanes: SPF45 Mouse mAb at 1/2000 dilutionLane 1: THP1 cells membrane fractionLane 2: Jurkat cells membrane fractionLysates/proteins at 20 ug per lane.Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilutionPredicted band size: 45 KDaObserved band size: 45 KDaBlocking/Dilution buffer : 1x TBST.)
Mouse anti-Human RBM17/SPF45 Monoclonal Antibody | anti-RBM17/SPF45 antibody
RBM17/SPF45 (514) Mouse mAb
IP: 10 tests
FC/FACS: 1:100-1:200
Western Blot (WB)
(All lanes: SPF45 Mouse mAb at 1/2000 dilutionLane 1: THP1 cells membrane fractionLane 2: Jurkat cells membrane fractionLysates/proteins at 20 ug per lane.Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilutionPredicted band size: 45 KDaObserved band size: 45 KDaBlocking/Dilution buffer : 1x TBST.)
Western Blot (WB)
(All lanes: SPF45 Mouse mAb at 1/2000 dilutionLane 1: THP1 cells membrane fractionLane 2: Jurkat cells membrane fractionLysates/proteins at 20 ug per lane.Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilutionPredicted band size: 45 KDaObserved band size: 45 KDaBlocking/Dilution buffer : 1x TBST.)
Immunoprecipitation (IP)
(SPF45 was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with MBS453137 of 10 ug. Western blot was performed from the immunoprecipitate using MBS435137 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction.Blocking and dilution buffer and concentration: 5% milk/TBST.)
Immunoprecipitation (IP)
(SPF45 was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with MBS453137 of 10 ug. Western blot was performed from the immunoprecipitate using MBS435137 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction.Blocking and dilution buffer and concentration: 5% milk/TBST.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling SPF45 with MBS435137 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling SPF45 with MBS435137 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an RNA binding protein. The encoded protein is part of the spliceosome complex and functions in the second catalytic step of mRNA splicing. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 9 and 15. [provided by RefSeq, Mar 2009]
Uniprot Description
Splice factor that binds to the single-stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.
Research Articles on RBM17/SPF45
Similar Products
Product Notes
The RBM17/SPF45 rbm17 (Catalog #AAA435137) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RBM17/SPF45 (514) Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RBM17/SPF45 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-1:5000 IP: 10 tests FC/FACS: 1:100-1:200. Researchers should empirically determine the suitability of the RBM17/SPF45 rbm17 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RBM17/SPF45, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
