Mouse anti-Human RASEF Monoclonal Antibody | anti-RASEF antibody

RASEF (Ras and EF-hand Domain-containing Protein, Ras-related Protein Rab-45, RAB45)

Cat. Num. AAA644441

• Gene Names: RASEF; RAB45
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RASEF; Monoclonal Antibody; RASEF (Ras and EF-hand Domain-containing Protein; Ras-related Protein Rab-45; RAB45); Anti -RASEF (Ras and EF-hand Domain-containing Protein; anti-RASEF antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 2D5
• Specificity: Recognizes human RASEF.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: NIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTGTNSKKSPQMKNCCNG

• Applicable Applications for anti-RASEF antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml
• Immunogen: Partial recombinant protein corresponding to aa631-740 from human RASEF (AAH23566) with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (38.1kD).)

Western Blot (WB)

(RASEF monoclonal antibody, Western Blot analysis of RASEF expression in K-562.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to RASEF on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 3ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged RASEF is ~0.3ng/ml as a capture antibody.)

Product Categories/Family for anti-RASEF antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 40255119
• NCBI GeneID: 158158
• NCBI Accession #: NP_689786.2
• NCBI GenBank Nucleotide #: NM_152573.3
• UniProt Accession #: Q8IZ41; Q96N04; A6NC29
• Molecular Weight: 82,879 Da
• NCBI Official Full Name: ras and EF-hand domain-containing protein
• NCBI Official Synonym Full Names: RAS and EF-hand domain containing
• NCBI Official Symbol: RASEF
• NCBI Official Synonym Symbols: RAB45
• NCBI Protein Information: ras and EF-hand domain-containing protein; ras-related protein Rab-45
• UniProt Protein Name: Ras and EF-hand domain-containing protein
• Protein Family: Ras and EF-hand domain-containing protein
• UniProt Gene Name: RASEF
• UniProt Synonym Gene Names: RAB45
• UniProt Entry Name: RASEF_HUMAN

NCBI Description

This gene is a member of the Rab family of GTPases that are involved in regulation of membrane traffic. The encoded protein contains an N-terminal EF-hand domain, a coiled-coil motif and a C-terminal Rab domain. A potential role as tumor suppressor has been indicated for this gene. [provided by RefSeq, Nov 2012]

Uniprot Description

RASEF: Binds predominantly GDP, and also GTP. Belongs to the small GTPase superfamily. Rab family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric, Rab; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 9q21.32

Cellular Component: perinuclear region of cytoplasm; endomembrane system

Molecular Function: GTPase activity; GDP binding; GTP binding; calcium ion binding

Biological Process: regulation of exocytosis; intracellular protein transport; metabolic process; protein secretion; Rab protein signal transduction; vesicle docking during exocytosis