Mouse anti-Human, Mouse RAB27B Monoclonal Antibody | anti-RAB27B antibody

RAB27B Antibody

Cat. Num. AAA9216852

• Gene Names: RAB27B; C25KG
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Synonyms: RAB27B; Monoclonal Antibody; RAB27B Antibody; Ras-related protein Rab-27B; C25KG; anti-RAB27B antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Specificity: This RAB27B antibody is generated from a mouse immunized with a recombinant protein of human RAB27B.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

• Applicable Applications for anti-RAB27B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:2000
• Function: Function Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion (PubMed:30771381). Plays a role in NTRK2/TRKB axonal anterograde transport by facilitating the association of NTRK2/TRKB with KLC1 (PubMed:21775604). May be involved in targeting uroplakins to urothelial apical membranes (By similarity).
• Cellular Location: Membrane; Lipid-anchor.
• Tissue Location: Expressed primarily in testis.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(WB - RAB27B Antibody AM8508b detail Anti-RAB27B Antibody at 1:2000 dilution + mouse stomach lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-RAB27B antibody

May be involved in targeting uroplakins to urothelial apical membranes.

Product Categories/Family for anti-RAB27B antibody

Crown Antibodies; Signal Transduction

References

Chen D.,et al.Biochem. Mol. Med. 60:27-37(1997). Chen D.,et al.Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases. Ramalho J.S.,et al.BMC Genet. 2:2-2(2001). Puhl H.L. III,et al.Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 5729997
• NCBI GeneID: 5874
• NCBI Accession #: NP_004154.2
• NCBI GenBank Nucleotide #: NM_004163.4
• UniProt Accession #: O00194; Q9BZB6; B2RAB0
• NCBI Official Full Name: ras-related protein Rab-27B
• NCBI Official Synonym Full Names: RAB27B, member RAS oncogene family
• NCBI Official Symbol: RAB27B
• NCBI Official Synonym Symbols: C25KG
• NCBI Protein Information: ras-related protein Rab-27B
• UniProt Protein Name: Ras-related protein Rab-27B
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB27B
• UniProt Entry Name: RB27B_HUMAN

NCBI Description

Members of the Rab protein family, including RAB27B, are prenylated, membrane-bound proteins involved in vesicular fusion and trafficking (Chen et al., 1997 [PubMed 9066979]).[supplied by OMIM, Nov 2010]

Uniprot Description

RAB27B: May be involved in targeting uroplakins to urothelial apical membranes. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 18q21.2

Cellular Component: apical plasma membrane; Golgi stack; melanosome; secretory granule; trans-Golgi network transport vesicle; zymogen granule membrane

Molecular Function: GDP binding; GTP binding; GTPase activity; myosin V binding; protein binding; protein domain specific binding

Biological Process: melanosome transport; positive regulation of exocytosis; protein transport; small GTPase mediated signal transduction