Rat anti-Human PTX3 Monoclonal Antibody | anti-PTX3 antibody

PTX3, Human, mAb MNB4

Cat. Num. AAA584258

• Gene Names: PTX3; TSG-14; TNFAIP5
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Western Blot
• Synonyms: PTX3; Monoclonal Antibody; Human; mAb MNB4; Pentraxin-related protein PTX3; Tumor necrosis factor-inducible gene 14 protein; TSG-14; anti-PTX3 antibody

• Host: Rat
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rat IgG2a
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 381

• Applicable Applications for anti-PTX3 antibody: F, Flow Cytometry (FC/FACS), Immunoassay (IA), Immunofluorescence (IF), Immunoprecipitation (IP), P, Western Blot (WB)
• Application Notes: Application Use: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50..
• Immunogen: Human recombinant PTX3
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-PTX3 antibody

Product Description: The monoclonal antibody MNB4 (previously known as clone 20) recognizes human pentraxin 3 (PTX3), belonging to the long pentraxin family. PTX3 is an acute-phase glycoprotein of ~45 kDa with glycosylation accounting for about 10% of its molecular weight. PTX3 has a complex oligomeric structure with protomers linked to each other by disulfide bonds. PTX3 expression is triggered by inflammatory cytokines, resulting in higher levels of circulating PTX3. Several cell types have been reported to produce PTX3, namely macrophages, endothelial cells, neutrophils and synoviocytes. PTX3 is involved in host defense against pathogen infection, in the regulation of the scavenger activity of macrophages and dendritic cells, and in modulation of complement activity by binding to C1q. Furthermore, PTX3 has been implicated in matrix deposition of cumulus cells. Moreover, PTX3 interacts with other biologically active molecules, causing their functional blockade. This has been demonstrated for fibroblast growth factor-2 (FGF-2), for which PTX3 acts as an inhibitor, leading to inhibition of angiogenesis. PTX3, like other pentraxins C-reactive protein (CRP) and serum amyloid P component (SAP), binds apoptotic cells and debris. PTX3 is useful as an early indicator of myocyte irreversible injury in ischemic cardiomyopathy. PTX3 is not only involved in inflammatory vessel diseases related to atherosclerosis, but also in pre-eclampsia and systemic small vessel ANCA-associated vasulitis, in which neutrophils are key players. The relationship between tissue damage and pentraxin generation is stringent in acute injuries. PTX3 tunes self-non-self discrimination and tissue repair due to the recognition of diverse ligands by PTX3 and through regulation of effector pathways.

References

1. Peri, G et al; PTX3, a prototypical long pentraxin, is an early indicator of acute myocardial infarction in humans. Circulation 2000, 102: 636 2. Fazzini, F et al; PTX3 in small-vessel vasculitides: an independent indicator of disease activity produced at sites of inflammation. Arthritis Rheum 2001, 44: 2841
3. Bussolati, B et al; The long pentraxin Ptx3 is synthesized in IgA glomerulonephritis and activates mesangial cells. J immunol 2003, 170: 1466
4. Inforzato, A et al; Structure and function of the long pentraxin PTX3 glycosidic moiety: fine-tuning of the interaction with C1q and complement activation. Biochemistry 2006, 45: 11540
5. Jaillon, S et al; The humoral pattern recognition receptor PTX3 is stored in neutrophil granules and localizes in extracellular traps. J Exp Med 2007, 204: 793

NCBI and Uniprot Product Information

• NCBI GI #: 167900484
• NCBI GeneID: 5806
• NCBI Accession #: NP_002843.2
• NCBI GenBank Nucleotide #: NM_002852.3
• Molecular Weight: 41,976 Da
• NCBI Official Full Name: pentraxin-related protein PTX3
• NCBI Official Synonym Full Names: pentraxin 3, long
• NCBI Official Symbol: PTX3
• NCBI Official Synonym Symbols: TSG-14; TNFAIP5
• NCBI Protein Information: pentraxin-related protein PTX3; pentraxin-3; TNF alpha-induced protein 5; pentaxin-related protein PTX3; tumor necrosis factor alpha-induced protein 5; tumor necrosis factor, alpha-induced protein 5; tumor necrosis factor-inducible protein TSG-14; tumor necrosis factor-inducible gene 14 protein; pentraxin-related gene, rapidly induced by IL-1 beta; pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor, alpha-induced protein 5
• UniProt Protein Name: Pentraxin-related protein PTX3
• Protein Family: Pentraxin-related protein
• UniProt Gene Name: PTX3
• UniProt Synonym Gene Names: TNFAIP5; TSG14; TNF alpha-induced protein 5; TSG-14
• UniProt Entry Name: PTX3_HUMAN

Uniprot Description

PTX3: Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self- components and female fertility.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q25

Cellular Component: extracellular space; extracellular region

Molecular Function: zymosan binding; complement component C1q binding; virion binding

Biological Process: positive regulation of nitric oxide biosynthetic process; negative regulation of virion penetration into host cell; positive regulation of phagocytosis; innate immune response; response to yeast; opsonization; inflammatory response