Rabbit anti-Human COT Polyclonal Antibody | anti-COT antibody

COT, CT (Cancer Osaka Thyroid Oncogene, c-COT, EST, ESTF, Mitogen-activated Protein Kinase Kinase Kinase 8, MAP3K8, MEKK8, Proto-oncogene c-Cot, Serine/threonine-protein Kinase Cot, Tumor Progression Locus 2, TPL2, TPL-2) (PE)

Cat. Num. AAA6470366

• Gene Names: MAP3K8; COT; EST; ESTF; TPL2; MEKK8; Tpl-2; c-COT
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: COT; Polyclonal Antibody; CT (Cancer Osaka Thyroid Oncogene; c-COT; EST; ESTF; Mitogen-activated Protein Kinase Kinase Kinase 8; MAP3K8; MEKK8; Proto-oncogene c-Cot; Serine/threonine-protein Kinase Cot; Tumor Progression Locus 2; TPL2; TPL-2) (PE); EC=2.7.11.25; anti-COT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human COT.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-COT antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 368-397 in the C-teminal region of human COT, UniProt Accession #P41279. Species sequence homology: mouse, rat
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot to detect COT in HeLa cell lysate usingMBS641654.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue reacted withMBS641654, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS641654 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted withMBS641654, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS641654 for immunohistochemistry.)

Product Categories/Family for anti-COT antibody

Antibodies; Protein Kinases

References

1. Sanchez-Gongora, E., et al., J. Biol. Chem. 275(40):31379-31386 (2000). 2. Aoki, M., et al., J. Biol. Chem. 268(30):22723-22732 (1993). 3. Chan, A.M., et al., Oncogene 8(5):1329-1333 (1993). 4. Miyoshi, J., et al., Mol. Cell. Biol. 11(8):4088-4096 (1991). 5. Aoki, M., et al., Oncogene 6(9):1515-1519 (1991).

NCBI and Uniprot Product Information

• NCBI GI #: 346644795
• NCBI GeneID: 1326
• NCBI Accession #: NP_001231063.1
• NCBI GenBank Nucleotide #: NM_001244134.1
• UniProt Accession #: P41279; Q14275; Q5T855; Q9HC81; A8K2Q5; D3DRX1
• Molecular Weight: 52,925 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 8
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 8
• NCBI Official Symbol: MAP3K8
• NCBI Official Synonym Symbols: COT; EST; ESTF; TPL2; MEKK8; Tpl-2; c-COT
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 8; proto-oncogene c-Cot; tumor progression locus 2; Ewing sarcoma transformant; cot (cancer Osaka thyroid) oncogene; proto-oncogene serine/threoine protein kinase
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 8
• UniProt Gene Name: MAP3K8
• UniProt Synonym Gene Names: COT; ESTF; TPL-2
• UniProt Entry Name: M3K8_HUMAN

NCBI Description

This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]

Uniprot Description

Cot: an oncogenic Ser/Thr kinase of the STE group. Activates IkappaB kinases, thus inducing the nuclear translocation of NF-kappaB. Promotes the production of TNF-alpha and IL-2 during T lymphocyte activation. Interacts with NFkB-p105. Overexpressed and amplified in breast tumors. Viral insertions induce rat lymphomas and mouse mammary carcinomas. Isolated as a transforming factor in two cell lines. Mediates LPS activation of macrophages. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Oncoprotein; EC 2.7.11.25; STE group; STE-Unique family

Chromosomal Location of Human Ortholog: 10p11.23

Cellular Component: cytoplasm; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; magnesium ion binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: activation of MAPKK activity; apoptosis; regulation of cell differentiation; T cell costimulation; MAPKKK cascade; cell cycle; protein amino acid phosphorylation

Disease: Lung Cancer