Mouse anti-Human pS2 Monoclonal Antibody | anti-pS2 antibody

pS2 (BCEI, Breast Cancer Estrogen Inducible Protein, Gastrointestinal Trefoil Protein, Gastrointestinal trefoil Protein pS2, HP1A, HPS2, pNR2, pNR-2, TFF1, Trefoil Factor 1) (HRP)

Cat. Num. AAA6124484

• Gene Names: TFF1; pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: pS2; Monoclonal Antibody; pS2 (BCEI; Breast Cancer Estrogen Inducible Protein; Gastrointestinal Trefoil Protein; Gastrointestinal trefoil Protein pS2; HP1A; HPS2; pNR2; pNR-2; TFF1; Trefoil Factor 1) (HRP); BCEI; Trefoil Factor 1; anti-pS2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: SPM573
• Specificity: Recognizes pS2. Species Crossreactivity: Human and Cynomolgus Monkey. Others-not known
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-pS2 antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IP: 0.5-1ug/500ug protein lysate; WB: 0.5-1.0ug/ml; IHC: 0.5-1.0ug/ml for 30 minutes at RT; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide of 28 amino acid residues corresponding to CFDDTVRGVPWCFYPNTIDVPPEEECEF (aa57-84) from the C-terminus of human pS2. Cellular Localization: Cytoplasmic
• Conjugate: HRP
• Positive Control: Spent medium of MCF-7 cells, treated with estrogen. Normal stomach. About 60% of breast carcinomas are positive for pS2, especially those that are also positive for estrogen and/or progesterone receptor.
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Categories/Family for anti-pS2 antibody

Antibodies; Trefoil Factor TFF

References

1. Williams R, et. al. Human Pathology, 1996, 27(12):1259-66.

NCBI and Uniprot Product Information

• NCBI GI #: 4507451
• NCBI GeneID: 7031
• NCBI Accession #: NP_003216.1
• NCBI GenBank Nucleotide #: NM_003225.2
• UniProt Accession #: P04155
• Molecular Weight: 9,150 Da
• NCBI Official Full Name: trefoil factor 1
• NCBI Official Synonym Full Names: trefoil factor 1
• NCBI Official Symbol: TFF1
• NCBI Official Synonym Symbols: pS2; BCEI; HPS2; HP1.A; pNR-2; D21S21
• NCBI Protein Information: trefoil factor 1; protein pS2; polypeptide P1.A; gastrointestinal trefoil protein pS2; breast cancer estrogen-inducible protein; breast cancer estrogen-inducible sequence
• UniProt Protein Name: Trefoil factor 1
• Protein Family: Inorganic pyrophosphatase
• UniProt Gene Name: TFF1
• UniProt Synonym Gene Names: BCEI; PS2; hP1.A
• UniProt Entry Name: TFF1_HUMAN

NCBI Description

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

Uniprot Description

TFF1: Stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. May inhibit the growth of calcium oxalate crystals in urine.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: extracellular space

Molecular Function: protein binding; growth factor activity

Biological Process: negative regulation of cell proliferation; response to peptide hormone stimulus; maintenance of gastrointestinal epithelium; digestion; carbohydrate metabolic process; cell differentiation; response to iron ion; response to estradiol stimulus