Human Thioredoxin (Trx) ELISA Kit | Trx elisa kit

Human Thioredoxin (Trx) ELISA Kit

Cat. Num. AAA2023995

• Gene Names: Txn1; Txn
• Reactivity: Human
• Synonyms: Thioredoxin (Trx); Human Thioredoxin (Trx) ELISA Kit; TXN; ADF; SASP; TRDX; TRX1; ATL-derived factor; Surface-associated sulphydryl protein; Trx elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Thioredoxin (Trx).; No significant cross-reactivity or interference between Thioredoxin (Trx) and analogues was observed.
• Sequence Length: 105

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 31.2-2,000pg/mL
• Sensitivity: < 12.7pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thioredoxin (Trx) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thioredoxin (Trx) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Trx elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thioredoxin (Trx). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thioredoxin (Trx). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thioredoxin (Trx), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thioredoxin (Trx) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for Trx elisa kit

Metabolic pathway; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 16758644
• NCBI GeneID: 116484
• NCBI Accession #: NP_446252.1
• NCBI GenBank Nucleotide #: NM_053800.3
• UniProt Accession #: P11232
• Molecular Weight: 11,673 Da
• NCBI Official Full Name: thioredoxin
• NCBI Official Synonym Full Names: thioredoxin 1
• NCBI Official Symbol: Txn1
• NCBI Official Synonym Symbols: Txn
• NCBI Protein Information: thioredoxin
• UniProt Protein Name: Thioredoxin
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: Txn
• UniProt Synonym Gene Names: Txn1; Trx
• UniProt Entry Name: THIO_RAT

NCBI Description

protein-disulfide reductase enzyme that is important for redox signalling during apoptosis and cell growth [RGD, Feb 2006]

Uniprot Description

TXN: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. In case of infection, interacts with S.typhimurium protein slrP. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA- binding activity in a redox-dependent manner. Up-regulated by ionizing radiation. Belongs to the thioredoxin family.

Protein type: Nuclear receptor co-regulator

Cellular Component: mitochondrion; cell soma; axon; dendrite; cytoplasm; extracellular region; cytosol; nucleus

Molecular Function: thioredoxin-disulfide reductase activity; enzyme binding; protein disulfide oxidoreductase activity; peptide disulfide oxidoreductase activity

Biological Process: response to drug; negative regulation of protein export from nucleus; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation; glycerol ether metabolic process; response to radiation; response to selenium ion; regulation of transcription, DNA-dependent; cell redox homeostasis; regulation of protein import into nucleus, translocation; response to axon injury; response to activity; positive regulation of DNA binding