Mouse anti-Human PRPK Monoclonal Antibody

PRPK (TP53-regulating Kinase, Nori-2, p53-related Protein Kinase, C20orf64, TP53RK)

Cat. Num. AAA649057

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PRPK; Monoclonal Antibody; PRPK (TP53-regulating Kinase; Nori-2; p53-related Protein Kinase; C20orf64; TP53RK); Anti -PRPK (TP53-regulating Kinase; anti-PRPK antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 4B9-1H3
• Specificity: Recognizes human TP53RK.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.

• Applicable Applications for anti-PRPK antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
• Immunogen: Recombinant corresponding to aa1-254 from human TP53RK with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(TP53RK monoclonal antibody Western Blot analysis of TP53RK expression in Jurkat.)

Testing Data

(Detection limit for recombinant GST tagged TP53RK is approximately 1ng/ml as a capture antibody.)

Related Product Information for anti-PRPK antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Product Categories/Family for anti-PRPK antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 14715574
• NCBI Official Full Name: PRPK mutant1

Uniprot Description

PRPK: Protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation. Belongs to the protein kinase superfamily.

Protein type: EC 2.7.11.1; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; Bud32 family

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: membrane; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; hydrolase activity; p53 binding; ATP binding

Biological Process: tRNA processing; protein amino acid phosphorylation