Western Blot (WB)
(The anti-PRPK Pab is used in Western blot to detect PRPK in mouse thymus tissue lysate.)
Rabbit anti-Human, mouse PRPK Polyclonal Antibody | anti-TP53RK antibody
PRPK Antibody (Center)
Gene Names
TP53RK; PRPK; BUD32; Nori-2; Nori-2p; dJ101A2; C20orf64
Reactivity
Human, mouse
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PRPK; Polyclonal Antibody; PRPK Antibody (Center); TP53-regulating kinase; Atypical serine/threonine protein kinase TP53RK; EKC/KEOPS complex subunit TP53RK; 36--; Nori-2; p53-related protein kinase; TP53RK; C20orf64; anti-TP53RK antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PRPK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 amino acids from the Central region of human PRPK.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
220-249
Sequence Length
253
Applicable Applications for anti-TP53RK antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(The anti-PRPK Pab is used in Western blot to detect PRPK in mouse thymus tissue lysate.)
Western Blot (WB)
(The anti-PRPK Pab is used in Western blot to detect PRPK in mouse thymus tissue lysate.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB)
(PRPK Antibody (V235) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the PRPK antibody detected the PRPK protein (arrow).)
Western Blot (WB)
(PRPK Antibody (V235) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the PRPK antibody detected the PRPK protein (arrow).)
Related Product Information for anti-TP53RK antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
Product Categories/Family for anti-TP53RK antibody
References
Miyoshi, A., et al., Biochem. Biophys. Res. Commun. 303(2):399-405 (2003).
Abe, Y., et al., J. Biol. Chem. 276(47):44003-44011 (2001).
Abe, Y., et al., J. Biol. Chem. 276(47):44003-44011 (2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28160
NCBI Official Full Name
TP53-regulating kinase
NCBI Official Synonym Full Names
TP53 regulating kinase
NCBI Official Symbol
TP53RK
NCBI Official Synonym Symbols
PRPK; BUD32; Nori-2; Nori-2p; dJ101A2; C20orf64
NCBI Protein Information
TP53-regulating kinase
UniProt Protein Name
TP53-regulating kinase
Protein Family
UniProt Gene Name
TP53RK
UniProt Synonym Gene Names
C20orf64; PRPK
UniProt Entry Name
PRPK_HUMAN
Uniprot Description
PRPK: Protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation. Belongs to the protein kinase superfamily.
Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; Other group; Bud32 family
Chromosomal Location of Human Ortholog: 20q13.2
Cellular Component: membrane; nucleus; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; hydrolase activity; p53 binding; ATP binding
Biological Process: tRNA processing; protein amino acid phosphorylation
Research Articles on TP53RK
Similar Products
Product Notes
The TP53RK tp53rk (Catalog #AAA9204654) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 220-249. The PRPK Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PRPK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the TP53RK tp53rk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRPK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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