Mouse anti-Human Progranulin Monoclonal Antibody

Progranulin (Proepithelin, PC Cell-derived Growth Factor)

Cat. Num. AAA601865

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Progranulin; Monoclonal Antibody; Progranulin (Proepithelin; PC Cell-derived Growth Factor); Anti -Progranulin (Proepithelin; anti-Progranulin antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 8H138
• Specificity: Recognizes human Progranulin. In direct ELISAs and Western Blots shows no crossreactivity with recombinant mouse Progranulin.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from 20mM Tris, pH 8.0, 300mM sodium chloride, 5% trehalose. Reconstitute with 200ul sterile PBS.

• Applicable Applications for anti-Progranulin antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Dilution: Western Blot: 1ug/ml detects Progranulin in recombinant human Progranulin under non-reducing conditions only.; Immunoprecipitation: 25ug/ml immunoprecipitates Progranulin from conditioned cell culture medium spiked with recombinant human Progranulin.
• Immunogen: Recombinant protein corresponding to aa18-aa593 from human Progranulin expressed in NSO cells (P28799).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Progranulin antibody

Progranulin is a ubiquitously expressed, 88kD, secreted glycoprotein. Structurally, it does not belong to any of the well established growth factor families. Human Progranulin is 593 amino acids (aa) in length and contains a 17aa signal sequence and 5 potential sites for N-linked glycosylation. Progranulin is involved in the regulation of cellular proliferation, as well as differentiation, development and pathological processes. It has been isolated as a differentially expressed gene during mesothelial differentiation, macrophage development, the development of rheumatoid arthritis and osteoarthritis, sexual differentiation of the brain and has also been shown to be a mediator of cartilage proliferation and of wound response and tissue repair. High levels of Progranulin expression have been found to be associated with several human cancers and are believed to contribute to tumorigenesis in breast cancer, clear cell renal carcinoma, invasive ovarian carcinoma, glioblastoma, adipocyte teratoma and multiple myeloma. In addition, mutations in the Progranulin gene are a cause of frontotemporal dementia, and increased expression of Progranulin is seen in activated microglia in many neurodegenerative diseases including Creutzfeldt-Jakob disease, motor neuron disease and Alzheimer's disease. Mutations in Progranulin causing neuro- degenerative disease indicate that Progranulin is important for neuronal survival.

Product Categories/Family for anti-Progranulin antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 251831945
• NCBI Official Full Name: progranulin