Testing Data
(Chemotaxis effect of Progranulin in THP-1 Cells: Cell migration of THP-1 monocytic leukemia cells was evaluated in disposable 24 well transwell polystyrene membrane with 8?m size pores. 0ng/ml, 10ng/ml, 100ng/ml and 250ng/ml of Progranulin were diluted in RPMI 1640 supplemented with 0.5% FBS respectively. In case boiled hProgranulin, proteins were boiled at 100°C for 10min. And then hProgranulin samples were added to the lower chamber. THP-1 cells (2 X 105 cells per well), in the same media with Progranulin, were added to the upper chamber. After 16 hours incubation at 37°C in 5% CO2 humidified atmosphere, migration cells in the lower chamber were counted. Migrated cells in two separate fields per well from triplicate wells were enumerated on a hemacytometer by means light microscopy.)
Progranulin Active Protein | GRN active protein
Human CellExp Progranulin, Human Recombinant
PBS containing at least 0.1% BSA should be used for further dilutions.
Testing Data
(Chemotaxis effect of Progranulin in THP-1 Cells: Cell migration of THP-1 monocytic leukemia cells was evaluated in disposable 24 well transwell polystyrene membrane with 8?m size pores. 0ng/ml, 10ng/ml, 100ng/ml and 250ng/ml of Progranulin were diluted in RPMI 1640 supplemented with 0.5% FBS respectively. In case boiled hProgranulin, proteins were boiled at 100°C for 10min. And then hProgranulin samples were added to the lower chamber. THP-1 cells (2 X 105 cells per well), in the same media with Progranulin, were added to the upper chamber. After 16 hours incubation at 37°C in 5% CO2 humidified atmosphere, migration cells in the lower chamber were counted. Migrated cells in two separate fields per well from triplicate wells were enumerated on a hemacytometer by means light microscopy.)
Testing Data
(Chemotaxis effect of Progranulin in THP-1 Cells: Cell migration of THP-1 monocytic leukemia cells was evaluated in disposable 24 well transwell polystyrene membrane with 8?m size pores. 0ng/ml, 10ng/ml, 100ng/ml and 250ng/ml of Progranulin were diluted in RPMI 1640 supplemented with 0.5% FBS respectively. In case boiled hProgranulin, proteins were boiled at 100°C for 10min. And then hProgranulin samples were added to the lower chamber. THP-1 cells (2 X 105 cells per well), in the same media with Progranulin, were added to the upper chamber. After 16 hours incubation at 37°C in 5% CO2 humidified atmosphere, migration cells in the lower chamber were counted. Migrated cells in two separate fields per well from triplicate wells were enumerated on a hemacytometer by means light microscopy.)
NCBI and Uniprot Product Information
NCBI Description
Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. [provided by RefSeq, Jul 2008]
Uniprot Description
GRN: Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD); also known as tau- negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease. Defects in GRN are the cause of neuronal ceroid lipofuscinosis type 11 (CLN11). A form of neuronal ceroid lipofuscinosis characterized by rapidly progressive visual loss due to retinal dystrophy, seizures, cerebellar ataxia, and cerebellar atrophy. Cognitive decline may also occur. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material. Belongs to the granulin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 17q21.32
Cellular Component: extracellular space; intracellular membrane-bound organelle; mitochondrion
Molecular Function: protein binding; growth factor activity; cytokine activity
Biological Process: blastocyst hatching; signal transduction; positive regulation of epithelial cell proliferation; embryo implantation
Disease: Frontotemporal Lobar Degeneration With Tdp43 Inclusions, Grn-related; Ceroid Lipofuscinosis, Neuronal, 11
Research Articles on GRN
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Product Notes
The GRN grn (Catalog #AAA844325) is an Active Protein produced from HEK 293 cells (aa 1-593) and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Progranulin can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE; HPLC. Researchers should empirically determine the suitability of the GRN grn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Signal peptide and human progranuli n (aa 1-593) are fused at the C-terminus to a FLAG®-tag. It is sometimes possible for the material contained within the vial of "Progranulin, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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