Rabbit anti-Human pro Caspase 9 Monoclonal Antibody | anti-CASP9 antibody

Anti-pro Caspase 9 Rabbit Monoclonal Antibody

Cat. Num. AAA179083

• Gene Names: CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Affinity-chromatography
• Synonyms: pro Caspase 9; Monoclonal Antibody; Anti-pro Caspase 9 Rabbit Monoclonal Antibody; Caspase-9; CASP-9; 3.4.22.62; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6; Caspase-9 subunit p35; Caspase-9 subunit p10; CASP9; MCH6; anti-CASP9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Purity/Purification: Affinity-chromatography
• Form/Format: Liquid
• Sequence Length: 333

• Applicable Applications for anti-CASP9 antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; FC/FACS: 1:50
• Immunogen: A synthesized peptide derived from human pro Caspase 9
• Tissue Specificity: Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
• Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of pro Caspase 9 expression in Jurkat cell lysate (MBS179083).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP9 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CASP9 )

Related Product Information for anti-CASP9 antibody

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP- ribose) polymerase (PARP).

Product Categories/Family for anti-CASP9 antibody

Cell Biology; Apoptosis; Intracellular; Caspases Etc; Caspases; Cancer; Invasion/Microenvironment; Cytochrome C; Metabolism; Pathways and Processes; Metabolism Processes; Cell Death; Apoptotic Markers

NCBI and Uniprot Product Information

• NCBI GI #: 14790124
• NCBI GeneID: 842
• NCBI Accession #: NP_001220.2
• NCBI GenBank Nucleotide #: NM_001229.4
• UniProt Accession #: P55211; O95348; Q53Y70; Q5JRU9; Q5UGI1; Q92852; Q9BQ62; Q9UEQ3; Q9UIJ8; B4E1A3
• Molecular Weight: 46281 MW
• NCBI Official Full Name: caspase-9 isoform alpha
• NCBI Official Synonym Full Names: caspase 9
• NCBI Official Symbol: CASP9
• NCBI Official Synonym Symbols: MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
• NCBI Protein Information: caspase-9
• UniProt Protein Name: Caspase-9
• Protein Family: Caspase
• UniProt Gene Name: CASP9
• UniProt Synonym Gene Names: MCH6; CASP-9; APAF-3; ICE-LAP6

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).