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Typical Testing Data/Standard Curve (for reference only)

Mouse Dipeptidyl Peptidase 9 (DPP9) ELISA Kit | DPP9 elisa kit

Mouse Dipeptidyl Peptidase 9 (DPP9) ELISA Kit

Gene Names
DPP9; DP9; DPLP9; DPRP2; DPRP-2
Reactivity
Mouse
Synonyms
Dipeptidyl Peptidase 9 (DPP9); Mouse Dipeptidyl Peptidase 9 (DPP9) ELISA Kit; DPRP2; DPLP9; Dipeptidylpeptidase 9; Dipeptidyl peptidase IV-related protein 2; Dipeptidyl peptidase-like protein 9; Dipeptidyl peptidase IX; DPP9 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Dipeptidyl Peptidase 9 (DPP9).
No significant cross-reactivity or interference between Dipeptidyl Peptidase 9 (DPP9) and analogues was observed.
Sequence Length
836
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates and Other Biological Fluids
Detection Range
78.12-5000pg/mL
Sensitivity
< 25pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Dipeptidyl Peptidase 9 (DPP9) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Dipeptidyl Peptidase 9 (DPP9) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for DPP9 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dipeptidyl Peptidase 9 (DPP9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dipeptidyl Peptidase 9 (DPP9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dipeptidyl Peptidase 9 (DPP9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dipeptidyl Peptidase 9 (DPP9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for DPP9 elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
95,013 Da
NCBI Official Full Name
dipeptidyl peptidase 9
NCBI Official Synonym Full Names
dipeptidyl-peptidase 9
NCBI Official Symbol
DPP9
NCBI Official Synonym Symbols
DP9; DPLP9; DPRP2; DPRP-2
NCBI Protein Information
dipeptidyl peptidase 9
UniProt Protein Name
Dipeptidyl peptidase 9
Protein Family
UniProt Gene Name
DPP9
UniProt Synonym Gene Names
DPRP2; DP9; DPRP-2; DPP IX; DPLP9
UniProt Entry Name
DPP9_HUMAN

NCBI Description

This gene encodes a protein that is a member of the S9B family in clan SC of the serine proteases. The protein has been shown to have post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Although the activity of this protein is similar to that of dipeptidyl peptidase 4 (DPP4), it does not appear to be membrane bound. In general, dipeptidyl peptidases appear to be involved in the regulation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer. Several transcript variants of this gene have been described but not fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

DPP9: Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. Belongs to the peptidase S9B family. DPPIV subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.14.5; Protease

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: membrane; cytoplasm; cytosol; nucleus

Molecular Function: identical protein binding; serine-type peptidase activity; aminopeptidase activity

Biological Process: proteolysis

Research Articles on DPP9

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Product Notes

The Mouse DPP9 dpp9 (Catalog #AAA2021595) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2021595 ELISA Kit recognizes Mouse DPP9. It is sometimes possible for the material contained within the vial of "Dipeptidyl Peptidase 9 (DPP9), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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