Rabbit anti-Human PRDX4 Monoclonal Antibody | anti-PRDX4 antibody

Anti-PRDX4 [SAIC-40A-24]

Cat. Num. AAA488565

• Gene Names: PRDX4; PRX-4; AOE372; AOE37-2; HEL-S-97n
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified antibody. Protein A affinity purified
• Synonyms: PRDX4; Monoclonal Antibody; Anti-PRDX4 [SAIC-40A-24]; Peroxiredoxin-4; anti-PRDX4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG, kappa
• Clone Number: SAIC-40A-24
• Specificity: Recognizes human PRDX4.
• Purity/Purification: Purified antibody. Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PRDX4 antibody: ELISA (EIA), Western Blot (WB), immuno Multiple Reaction Monitoring (immuno-MRM)
• Application Notes: Original data characterizing this antibody may be found here.
• PMID: 25512614
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Flow Cytometry (FC/FACS)

(Flow cytometry using the Anti-PRDX4 antibody SAIC-40A-24 (MBS488565). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG version of SAIC-40A-24 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)

Western Blot (WB)

(Western Blot using anti-PRDX4 antibody SAIC-40A-24 (MBS488565). Human liver(A) and human pancreas(B) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-40A-24 () at 0.001ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)

Related Product Information for anti-PRDX4 antibody

Recombinant monoclonal antibody to PRDX4. Recombinant Platform with variable regions (i.e. specificity) from the B-cell cDNA library SAIC-40A-24.
Peptide "QITLNDLPVGR", derived from the cell redox-regulator Peroxiredoxin-4, conjugated to KLH.

Product Categories/Family for anti-PRDX4 antibody

immunoMRM

References

Schoenherr et al. Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Mol Cell Proteomics
Note on publication: Describes generation a panel of recombinant antibodies using synthetic peptides of human proteins as well as validation using ELISA and multiple reaction monitoring-mass spectrometry.

NCBI and Uniprot Product Information

• NCBI GI #: 3024727
• NCBI GeneID: 10549
• UniProt Accession #: Q13162; Q6FHT3
• Molecular Weight: 271
• NCBI Official Full Name: Peroxiredoxin-4
• NCBI Official Synonym Full Names: peroxiredoxin 4
• NCBI Official Symbol: PRDX4
• NCBI Official Synonym Symbols: PRX-4; AOE372; AOE37-2; HEL-S-97n
• NCBI Protein Information: peroxiredoxin-4; prx-IV; peroxiredoxin IV; antioxidant enzyme AOE372; thioredoxin peroxidase AO372; thioredoxin peroxidase (antioxidant enzyme); thioredoxin-dependent peroxide reductase A0372; epididymis secretory sperm binding protein Li 97n
• UniProt Protein Name: Peroxiredoxin-4
• Protein Family: Peroxiredoxin
• UniProt Gene Name: PRDX4
• UniProt Synonym Gene Names: AOE37-2; Prx-IV
• UniProt Entry Name: PRDX4_HUMAN

NCBI Description

The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB. [provided by RefSeq, Jul 2008]

Uniprot Description

PRDX4: Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. Belongs to the AhpC/TSA family.

Protein type: Secreted; Mitochondrial; Oxidoreductase; EC 1.11.1.15; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xp22.11

Cellular Component: extracellular space; smooth endoplasmic reticulum; mitochondrion; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; thioredoxin peroxidase activity

Biological Process: extracellular matrix organization and biogenesis; protein maturation via protein folding; 4-hydroxyproline metabolic process; cell redox homeostasis; male gonad development; spermatogenesis; I-kappaB phosphorylation