Flow Cytometry (FC/FACS)
(Flow cytometry using the Anti-PRDX4 antibody SAIC-40C-8 (MBS488566). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG version of SAIC-40C-8 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Rabbit anti-Human PRDX4 Monoclonal Antibody | anti-PRDX4 antibody
Anti-PRDX4 [SAIC-40C-8]
Flow Cytometry (FC/FACS)
(Flow cytometry using the Anti-PRDX4 antibody SAIC-40C-8 (MBS488566). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG version of SAIC-40C-8 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow cytometry using the Anti-PRDX4 antibody SAIC-40C-8 (MBS488566). Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG version of SAIC-40C-8 (, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Western Blot (WB)
(Western Blot using anti-PRDX4 antibody SAIC-40C-8 (MBS488566). HEK293 cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-40C-8 () at 0.001ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Western Blot (WB)
(Western Blot using anti-PRDX4 antibody SAIC-40C-8 (MBS488566). HEK293 cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-40C-8 () at 0.001ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Peptide "LVQAFQYTDK" derived from the cell redox-regulator Peroxiredoxin-4, conjugated to KLH.
Note on publication: Describes generation a panel of recombinant antibodies using synthetic peptides of human proteins as well as validation using ELISA and multiple reaction monitoring-mass spectrometry.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB. [provided by RefSeq, Jul 2008]
Uniprot Description
PRDX4: Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. Belongs to the AhpC/TSA family.
Protein type: Secreted; Mitochondrial; Oxidoreductase; EC 1.11.1.15; Secreted, signal peptide
Chromosomal Location of Human Ortholog: Xp22.11
Cellular Component: extracellular space; smooth endoplasmic reticulum; mitochondrion; nucleus; cytosol
Molecular Function: protein binding; protein homodimerization activity; thioredoxin peroxidase activity
Biological Process: extracellular matrix organization and biogenesis; protein maturation via protein folding; 4-hydroxyproline metabolic process; cell redox homeostasis; male gonad development; spermatogenesis; I-kappaB phosphorylation
Research Articles on PRDX4
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Product Notes
The PRDX4 prdx4 (Catalog #AAA488566) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PRDX4 [SAIC-40C-8] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRDX4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), immuno Multiple Reaction Monitoring (immuno-MRM). Original data characterizing this antibody may be found here. Researchers should empirically determine the suitability of the PRDX4 prdx4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRDX4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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