Mouse anti-Human CRBN Polyclonal Antibody | anti-crbn antibody

CRBN (Protein Cereblon, AD-006, DKFZp781K0715, MGC27358)

Cat. Num. AAA649923

• Gene Names: crbn; zgc:92404
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: CRBN; Polyclonal Antibody; CRBN (Protein Cereblon; AD-006; DKFZp781K0715; MGC27358); Anti -CRBN (Protein Cereblon; anti-crbn antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CRBN.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MAGEGDQQDAAHNMGNHLPLLPAESEEEDEMEVEDQDSKEAKKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQLFHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQDFGIEIVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQLESLNKCQIFPSKPVSREDQCSYKWWQKYQKRKFHCANLTSWPRWLYSLYDAETLMDRIKKQLREWDENLKDDSLPSNPIDFSYRVAACLPIDDVLRIQLLKIGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYVNPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLPTIPDTEDEISPDKVILCL

• Applicable Applications for anti-crbn antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human CRBN, aa1-442 (NP_057386.2).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of CRBN expression in transfected 293T cell line by MBS649923. Lane 1: CRBN transfected lysate (48.62kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-crbn antibody

Component of some DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8. In the complex, may act as a substrate receptor. Regulates the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1.

Product Categories/Family for anti-crbn antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 182890566
• NCBI GeneID: 445491
• UniProt Accession #: Q68EH9
• Molecular Weight: 49,095 Da
• NCBI Official Full Name: Crbn protein
• NCBI Official Synonym Full Names: cereblon
• NCBI Official Symbol: crbn
• NCBI Official Synonym Symbols: zgc:92404
• NCBI Protein Information: protein cereblon; zcrbn
• UniProt Protein Name: Protein cereblon
• Protein Family: Protein cereblon
• UniProt Gene Name: crbn
• UniProt Synonym Gene Names: zcrbn
• UniProt Entry Name: CRBN_DANRE

Uniprot Description

Function: Component of some DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8. In the complex, may act as a substrate receptor. May also be involved in memory and learning. Ref.2

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Component of a DCX (DDB1-CUL4-X-box) protein ligase complex

By similarity.

Subcellular location: Cytoplasm

By similarity. Nucleus

By similarity.

Tissue specificity: Highly expressed in brain, head, vasculature otic vesicles and developing pectoral fins. Ref.2

Disruption phenotype: Specific defects in fin and otic vesicle development. Ref.2

Miscellaneous: Specifically binds thalidomide. Thalidomide teratogenic activity may be a consequence of crbn-binding. Overexpression of a mutant protein abolishing thalidomide-binding does not affect otic vesicle size (Ref.2).

Sequence similarities: Belongs to the CRBN family.Contains 1 Lon domain.