Human Collagen Type IV Alpha 3 (COL4a3) ELISA Kit | COL4a3 elisa kit

Human Collagen Type IV Alpha 3 (COL4a3) ELISA Kit

Cat. Num. AAA167571

• Reactivity: Human
• Synonyms: Collagen Type IV Alpha 3 (COL4a3); Human Collagen Type IV Alpha 3 (COL4a3) ELISA Kit; COL4a3 elisa kit

• Reactivity: Human
• Sequence Length: 199

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.2ng/ml - 70ng/ml
• Sensitivity: 0.12ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL4a3 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Collagen Type IV Alpha 3 (also known as COL4a3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human COL4a3 antibody. COL4a3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human COL4a3 Antibody is added and binds to COL4a3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated COL4a3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human COL4a3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 409107
• NCBI GeneID: 1285
• Molecular Weight: 158,336 Da
• NCBI Official Full Name: collagen type IV alpha 3, partial
• NCBI Official Synonym Full Names: collagen type IV alpha 3 chain
• NCBI Official Symbol: COL4A3
• NCBI Protein Information: collagen alpha-3(IV) chain
• UniProt Protein Name: Collagen alpha-3(IV) chain
• Protein Family: Collagen
• UniProt Gene Name: COL4A3

NCBI Description

Type IV collagen, the major structural component of basement membranes, is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome, autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jun 2010]

Uniprot Description

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.