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Western Blot (WB) (Western blot detection of PP2A alpha and beta in Hela, MCF7, C6 and 3T3 cell lysates using PP2A alpha and beta mouse mAb (1:2000 diluted).Predicted band size:36KDa.Observed band size:36KDa.)

PP2A alpha and beta Monoclonal Antibody | anti-PP2Abeta antibody

Anti-PP2A alpha and beta Mouse mAb

Gene Names
PPP2CB; PP2CB; PP2Abeta
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Affinity Purified
Synonyms
PP2A alpha and beta; Monoclonal Antibody; Anti-PP2A alpha and beta Mouse mAb; PP2A beta; PP2A-beta; PP2AB_HUMAN; PP2Abeta; PP2CB; Ppp2cb; Protein phosphatase 2 (formerly 2A); catalytic subunit; beta isoform; Protein phosphatase 2 catalytic subunit beta isozyme; Protein phosphatase 2; Protein phosphatase 2A catalytic subunit beta isoform; Protein phosphatase type 2A catalytic subunit; Serine/threonine protein phosphatase 2A catalytic subunit beta; Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform; anti-PP2Abeta antibody
Ordering
For Research Use Only!
Host
Mouse
Source: Human
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
2G2-H6-G11-E8
Purity/Purification
Affinity Purified
Form/Format
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Sequence Length
309
Applicable Applications for anti-PP2Abeta antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:2000
Immunogen
Purified recombinant human full length PP2A beta protein expressed in E Coli
Sensitivity
This antibody detects endogenous levels of PP2A alpha and PP2A beta.
Sequence Similarities
Belongs to the PPP phosphatase family.PP-1 subfamily.
Subunit Structure
Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD.Interacts with TBCD by similarity.PP2A consists of a common heterodimeric core enzyme (composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65) (subunit A)) that associates with a variety of regulatory subunits.Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.Binds PPME1.May indirectly interact with SGOL1, most probably through regulatory B56 subunits.Interacts with CTTNBP2NL.

Western Blot (WB)

(Western blot detection of PP2A alpha and beta in Hela, MCF7, C6 and 3T3 cell lysates using PP2A alpha and beta mouse mAb (1:2000 diluted).Predicted band size:36KDa.Observed band size:36KDa.)

Western Blot (WB) (Western blot detection of PP2A alpha and beta in Hela, MCF7, C6 and 3T3 cell lysates using PP2A alpha and beta mouse mAb (1:2000 diluted).Predicted band size:36KDa.Observed band size:36KDa.)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of Hela cell lysates using PP2A alpha and beta mouse mAb.)

Immunoprecipitation (IP) (Immunoprecipitation analysis of Hela cell lysates using PP2A alpha and beta mouse mAb.)
Related Product Information for anti-PP2Abeta antibody
Entrez Summary: This gene encodes the phosphatase 2A catalytic subunit.Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division.It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits.This gene encodes a beta isoform of the catalytic subunit.[provided by RefSeq, Mar 2010]
UniPort Summary Function: PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36
NCBI Official Full Name
serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
NCBI Official Synonym Full Names
protein phosphatase 2 catalytic subunit beta
NCBI Official Symbol
PPP2CB
NCBI Official Synonym Symbols
PP2CB; PP2Abeta
NCBI Protein Information
serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
UniProt Protein Name
Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform
UniProt Gene Name
PPP2CB
UniProt Synonym Gene Names
PP2A-beta

NCBI Description

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. [provided by RefSeq, Mar 2010]

Uniprot Description

PPP2CB: one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits.

Protein type: EC 3.1.3.16; Motility/polarity/chemotaxis; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: cytosol; protein phosphatase type 2A complex

Molecular Function: protein binding; protein serine/threonine phosphatase activity

Biological Process: protein amino acid dephosphorylation

Research Articles on PP2Abeta

Similar Products

Product Notes

The PP2Abeta ppp2cb (Catalog #AAA475192) is an Antibody produced from Mouse Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-PP2A alpha and beta Mouse mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PP2A alpha and beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:2000. Researchers should empirically determine the suitability of the PP2Abeta ppp2cb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PP2A alpha and beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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