Mouse Polyglutamylation Modification Monoclonal Antibody
Polyglutamylation Modification (Biotin) (Polyglutamylated Tubulin, Glutamylated Tubulin, Postranslational Protein Glutamylation)
Applications
Immunocytochemistry, Immunoprecipitation, Western Blot, Immunohistochemistry
Purity
Purified by Protein G affinity chromatography from concentratedhybridoma tissue culture supernatant.
Synonyms
Polyglutamylation Modification; Monoclonal Antibody; Polyglutamylation Modification (Biotin) (Polyglutamylated Tubulin; Glutamylated Tubulin; Postranslational Protein Glutamylation); Anti -Polyglutamylation Modification (Biotin) (Polyglutamylated Tubulin; anti-Polyglutamylation Modification antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
GT335
Specificity
Recognizes the posttranslational modification (poly)glutamylation on proteins. Reacts with polyglutamylated alpha- and beta-tubulin.
Purity/Purification
Purified by Protein G affinity chromatography from concentratedhybridoma tissue culture supernatant.
Form/Format
Supplied as a liquid in PBS, 0.02% sodium azide. Labeled with Biotin.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Polyglutamylation Modification antibody
Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Immunohistochemistry. (IHC)
Application Notes
Immunocytochemistry: 1:2000
Immunohistochemistry (paraffin sections): 1:1000
Western Blot: 1:4000
Optimal dilutions to be determined by researcher.
Immunohistochemistry (paraffin sections): 1:1000
Western Blot: 1:4000
Optimal dilutions to be determined by researcher.
Immunogen
Octapeptide EGEGE*EEG, modified by the addition of two glutamyl units onto the fifth E (indicated by an asterisk).
Grade
Affinity Purified
Preparation and Storage
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Polyglutamylation Modification antibody
Polyglutamylation is a post-translational modification in which glutamate side chains of variable lengths are formed on the modified protein. It is evolutionarily conserved from and the most prominent substrate is tubulin, the microtubule (MT) building block. Polyglutamylation has been proposed to be involved in the functional adaptation of MTs, as it occurs within the carboxy-terminal tubulin tails that participate directly in the binding of many structural and motor MT-associated proteins. The recent identification of new substrates of polyglutamylation indicates that this post-translational modification could be a potential regulator of diverse cellular processes and involved in cell cycle and cell proliferation.
Product Categories/Family for anti-Polyglutamylation Modification antibody
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Product Notes
The Polyglutamylation Modification (Catalog #AAA6009439) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Polyglutamylation Modification can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Immunohistochemistry. (IHC). Immunocytochemistry: 1:2000 Immunohistochemistry (paraffin sections): 1:1000 Western Blot: 1:4000 Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the Polyglutamylation Modification for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Polyglutamylation Modification, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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