Mouse POLR2J2 Monoclonal Antibody | anti-POLR2J2 antibody

POLR2J2 antibody

Cat. Num. AAA832295

• Gene Names: POLR2J2; HRPB11B; RPB11b1
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: POLR2J2 antibody was purified by affinity chromatography.
• Synonyms: POLR2J2; Monoclonal Antibody; POLR2J2 antibody; Monoclonal POLR2J2; Anti-POLR2J2; RPB11b1; DNA-directed RNA polymerase II subunit J2; RNA polymerase II subunit B11-b1; HRPB11B; MGC105050; MGC54043; RPB11; POLRJ 2; POLRJ-2; anti-POLR2J2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1C6
• Purity/Purification: POLR2J2 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 115

• Applicable Applications for anti-POLR2J2 antibody: Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: FC: 1:100; WB: 1:2000
• Biological Significance: POLR2J2 is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22. 1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymeraseII polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizingmessenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially expressisoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants arespliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay(NMD).
• Cross-Reactivity: Human
• Immunogen: POLR2J2 antibody was raised in mouse using a full length recombinant protein of human POLR2J2 (NP_116581) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Hela cells using POLR2J2 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant POLR2J2 protein (Right) or empty vector (Left) detected with POLR2J2 antibody)

Related Product Information for anti-POLR2J2 antibody

Mouse monoclonal POLR2J2 antibody

Product Categories/Family for anti-POLR2J2 antibody

DNA & RNA

NCBI and Uniprot Product Information

• NCBI GI #: 62739171
• NCBI GeneID: 246721
• NCBI Accession #: NP_116581
• NCBI GenBank Nucleotide #: NM_032959.5
• UniProt Accession #: Q9GZM3; Q9H1A8; F6W009
• Molecular Weight: 12,733 Da
• NCBI Official Full Name: DNA-directed RNA polymerase II subunit RPB11-b1
• NCBI Official Synonym Full Names: polymerase (RNA) II (DNA directed) polypeptide J2
• NCBI Official Symbol: POLR2J2
• NCBI Official Synonym Symbols: HRPB11B; RPB11b1
• NCBI Protein Information: DNA-directed RNA polymerase II subunit RPB11-b1
• UniProt Protein Name: DNA-directed RNA polymerase II subunit RPB11-b1
• Protein Family: DNA-directed RNA polymerase
• UniProt Gene Name: POLR2J2
• UniProt Synonym Gene Names: RNA polymerase II subunit B11-b1; RPB11b1
• UniProt Entry Name: RPB1B_HUMAN

NCBI Description

This gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq, Jul 2008]

Uniprot Description

POLR2J2: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft. Belongs to the archaeal RpoL/eukaryotic RPB11/RPC19 RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transferase

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: nucleus

Molecular Function: protein dimerization activity; DNA binding; DNA-directed RNA polymerase activity

Biological Process: transcription, DNA-dependent