Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant POLR2E protein using POLR2E antibody)
Mouse POLR2E Monoclonal Antibody | anti-POLR2E antibody
POLR2E antibody
Applications
Immunofluorescence, Western Blot
Purity
POLR2E antibody was purified by affinity chromatography.
Synonyms
POLR2E; Monoclonal Antibody; POLR2E antibody; Monoclonal POLR2E; Anti-POLR2E; DNA-directed RNA polymerase II subunit E; RNA polymerases I II and III subunit ABC1; XAP4; RPABC1; RPB5; hRPB25; POLRE 2; POLRE-2; anti-POLR2E antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3C5
Purity/Purification
POLR2E antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
210
Applicable Applications for anti-POLR2E antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes
IF: 1:100
WB: 1:500-2000
WB: 1:500-2000
Biological Significance
This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virustransactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and thepolymerase can occur through this subunit. A pseudogene is located on chromosome 11.
Cross-Reactivity
Human
Immunogen
POLR2E antibody was raised in mouse using a full length recombinant protein of human POLR2E (NP_002686) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant POLR2E protein using POLR2E antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant POLR2E protein using POLR2E antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant POLR2E protein (Right) or empty vector (Left) detected with POLR2E antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant POLR2E protein (Right) or empty vector (Left) detected with POLR2E antibody)
Related Product Information for anti-POLR2E antibody
Mouse monoclonal POLR2E antibody
Product Categories/Family for anti-POLR2E antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
POLR2E, partial
Similar Products
Product Notes
The POLR2E (Catalog #AAA838440) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's POLR2E can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). IF: 1:100 WB: 1:500-2000. Researchers should empirically determine the suitability of the POLR2E for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLR2E, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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