Human Laminin (LN) ELISA Kit | LN elisa kit

Human Laminin (LN) ELISA Kit

Cat. Num. AAA9715467

• Gene Names: LAMC2; B2T; CSF; EBR2; BM600; EBR2A; LAMB2T; LAMNB2
• Reactivity: Human
• Synonyms: Laminin (LN); Human Laminin (LN) ELISA Kit; LN elisa kit

• Reactivity: Human
• Specificity: Human Laminin (LN) ELISA Kit has high sensitivity and excellent specificity for detection of Human LN. No significant cross-reactivity or interference between Human LN and analogues was observed.
• Sequence Length: 1111

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Laminin (LN) Standard Curve.)

Related Product Information for LN elisa kit

Description: This Human Laminin (LN) ELISA Kit employs a two-site sandwich ELISA to quantitate LN in samples. An antibody specific for LN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Laminin, a protein network foundation for most cells and organs. The laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration, adhesion as well as phenotype and survival. Laminins are large trimeric proteins that contain an alpha-chain, a beta-chain and a gamma-chain, found in five, three and three genetic variants, respectively. The laminin molecules are named according to their chain composition. Thus, laminin-511 contains alpha5, beta1 and gamma1 chains. Fourteen other chain combinations have been identified in vivo. The trimeric proteins form a cross, giving a structure that can bind to other cell membrane and extracellular matrix molecules. The three shorter arms are particularly good at binding to other laminin molecules, which allows them to form sheets. The long arm is capable of binding to cells, which helps anchor organized tissue cells to the membrane.

NCBI and Uniprot Product Information

• NCBI GI #: 34232
• NCBI GeneID: 3918
• NCBI Official Full Name: Laminin
• NCBI Official Synonym Full Names: laminin subunit gamma 2
• NCBI Official Symbol: LAMC2
• NCBI Official Synonym Symbols: B2T; CSF; EBR2; BM600; EBR2A; LAMB2T; LAMNB2
• NCBI Protein Information: laminin subunit gamma-2

NCBI Description

Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature. [provided by RefSeq, Aug 2011]