Mouse anti-Human GPN1 / XAB1 Monoclonal Antibody | anti-GPN1 antibody

Mouse Monoclonal [clone 3E1] (IgG1,k) to Human GPN1 / XAB1

Cat. Num. AAA245623

• Gene Names: GPN1; XAB1; MBDIN; NTPBP; RPAP4; ATPBD1A
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: GPN1 / XAB1; Monoclonal Antibody; Mouse Monoclonal [clone 3E1] (IgG1; k) to Human GPN1 / XAB1; Anti-GPN1 / XAB1 Antibody (clone 3E1) IHC-plus; GPN1; ATP(GTP)-binding protein; ATPBD1A; GPN-loop GTPase 1; MBD2-interacting protein; RPAP4; XPA binding protein 1; GTPase; MBDIN; NTPBP; XAB1; XPA-binding protein 1; Human GPN1; anti-GPN1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3E1
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 388

• Applicable Applications for anti-GPN1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB; Usage: Western Blot (Cell lysate) - positive control HL-60. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
• Target Species: Human
• Immunogen Description: XAB1 (AAH07451, 9 a.a. ~ 119 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: GPN1 / XAB1 antibody was raised against xAB1 (AAH07451, 9 a.a. ~ 119 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-GPN1 / XAB1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-GPN1 / XAB1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to XAB1 on HeLa cell (antibody concentration 10 ug/ml).)

Western Blot (WB)

(Western blot of XAB1 expression in transfected 293T cell line by XAB1 monoclonal antibody, clone 3E1.)

Western Blot (WB)

(XAB1 monoclonal antibody clone 3E1 Western blot of XAB1 expression in HL-60.)

ELISA (EIA)

(Detection limit for recombinant GST tagged XAB1 is approximately 3 ng/ml as a capture antibody.)

Product Categories/Family for anti-GPN1 antibody

Family: Ras GTPase superfamily IPR001806

NCBI and Uniprot Product Information

• NCBI GI #: 223005897
• NCBI GeneID: 11321
• NCBI Accession #: NP_009197.2
• NCBI GenBank Nucleotide #: NM_007266.3
• UniProt Accession #: Q9HCN4; O76004; B4DQJ5; B4DQM4; B4DXU4; B5MBZ5
• Molecular Weight: 43,345 Da
• NCBI Official Full Name: GPN-loop GTPase 1 isoform a
• NCBI Official Synonym Full Names: GPN-loop GTPase 1
• NCBI Official Symbol: GPN1
• NCBI Official Synonym Symbols: XAB1; MBDIN; NTPBP; RPAP4; ATPBD1A
• NCBI Protein Information: GPN-loop GTPase 1; ATP(GTP)-binding protein; MBD2-interacting protein; RNAPII-associated protein 4; XPA binding protein 1, GTPase; RNA polymerase II associated protein 4
• UniProt Protein Name: GPN-loop GTPase 1
• UniProt Gene Name: GPN1
• UniProt Synonym Gene Names: MBDin
• UniProt Entry Name: GPN1_HUMAN

NCBI Description

This gene encodes a guanosine triphosphatase enzyme. The encoded protein may play a role in DNA repair and may function in activation of transcription. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2009]

Uniprot Description

GPN1: Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. May be involved in nuclear localization of XPA. Belongs to the GPN-loop GTPase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: cytoplasm; nucleus

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: metabolic process