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Western Blot (WB)
(Western blot analysis of PINCH on human PINCH recombinant protein using anti-PINCH antibody at 1/1, 000 dilution.)
Mouse anti-Human PINCH Monoclonal Antibody | anti-PINCH antibody
PINCH Antibody
Gene Names
LIMS1; PINCH; PINCH1; PINCH-1
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
PINCH; Monoclonal Antibody; PINCH Antibody; 2310016J22Rik antibody; 4921524A02Rik antibody; AI507642 antibody; AU021743 antibody; AW551584 antibody; C430041B13Rik antibody; LIM and senescent cell antigen like domains 1 antibody; LIM and senescent cell antigen-like-containing domain protein 1 antibody; LIM zinc finger domain containing 1 antibody; LIM-type zinc finger domains 1 antibody; Lims1 antibody; LIMS1_HUMAN antibody; Lims1l antibody; Particularly interesting new Cys His protein 1 antibody; Particularly interesting new Cys His protein antibody; Particularly interesting new Cys-His protein 1 antibody; PINCH 1 antibody; PINCH antibody; PINCH-1 antibody; PINCH1 antibody; Renal carcinoma antigen NY REN 48 antibody; Renal carcinoma antigen NY-REN-48 antibody; senescent cell antigen antibody; wu:fc32c03 antibody; zgc:112533 antibody; anti-PINCH antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
B9-G7
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PINCH antibody
Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:1000
ICC: 1:100-1:500
FC/FACS: 1:100-1:200
ICC: 1:100-1:500
FC/FACS: 1:100-1:200
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of PINCH on human PINCH recombinant protein using anti-PINCH antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of PINCH on human PINCH recombinant protein using anti-PINCH antibody at 1/1, 000 dilution.)
Western Blot (WB)
(Western blot analysis of PINCH on different cell lysates using anti-PINCH antibody at 1/1, 000 dilution. Positive control: Lane 1: A549 Lane 2: Jurkat Lane 3: Hela)
Western Blot (WB)
(Western blot analysis of PINCH on different cell lysates using anti-PINCH antibody at 1/1, 000 dilution. Positive control: Lane 1: A549 Lane 2: Jurkat Lane 3: Hela)
Immunocytochemistry (ICC)
(ICC staining PINCH (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining PINCH (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells with PINCH antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells with PINCH antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).)
Related Product Information for anti-PINCH antibody
Pinch, also designated particularly interesting new Cys-His protein or NY-REN-48, is a focal adhesion protein that is a component of the ILK-PINCH complex. This complex is a major part of the growth factor and integrin signaling pathway. Pinch is involved in cell differentiation, proliferation and survival by acting as an effector of integrin and growth factor signaling. It is a cytoplasmic protein expressed in most tissues and consists of five LIM domains, a nuclear localization signal and a nuclear export signal. The PINCH-1/ILK complex is regulated by a PINCH-1 related protein PINCH-2, which also forms a complex with ILK.
Product Categories/Family for anti-PINCH antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,571 Da
NCBI Official Full Name
LIM and senescent cell antigen-like-containing domain protein 1 isoform c
NCBI Official Synonym Full Names
LIM and senescent cell antigen-like domains 1
NCBI Official Symbol
LIMS1
NCBI Official Synonym Symbols
PINCH; PINCH1; PINCH-1
NCBI Protein Information
LIM and senescent cell antigen-like-containing domain protein 1; particularly interesting new Cys-His protein 1; renal carcinoma antigen NY-REN-48
UniProt Protein Name
LIM and senescent cell antigen-like-containing domain protein 1
UniProt Gene Name
LIMS1
UniProt Synonym Gene Names
PINCH; PINCH1; PINCH-1
UniProt Entry Name
LIMS1_HUMAN
Similar Products
Product Notes
The PINCH lims1 (Catalog #AAA9435329) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PINCH Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PINCH can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:500-1:1000 ICC: 1:100-1:500 FC/FACS: 1:100-1:200. Researchers should empirically determine the suitability of the PINCH lims1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PINCH, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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