Rabbit LIMS1 Polyclonal Antibody | anti-LIMS1 antibody

LIMS1 antibody

Cat. Num. AAA9402505

• Gene Names: LIMS1; PINCH; PINCH1; PINCH-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: LIMS1; Polyclonal Antibody; LIMS1 antibody; PINCH; PINCH1; PINCH-1; anti-LIMS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total LIMS1 antibody.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 329

• Applicable Applications for anti-LIMS1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunofluorescence: 1:50 - 1:100
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human LIMS1.
• Target Name: LIMS1
• Preparation and Storage: Store at -20 degree C

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cell using LIMS1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-LIMS1 antibody

The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-LIMS1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 301601605
• NCBI GeneID: 3987
• NCBI Accession #: NP_001180411.1
• NCBI GenBank Nucleotide #: NM_001193482.1
• UniProt Accession #: P48059; Q53TE0; Q9BS44; B2RAJ4; B7Z483; B7Z7R3; B7Z907
• Molecular Weight: 41,571 Da
• NCBI Official Full Name: LIM and senescent cell antigen-like-containing domain protein 1 isoform c
• NCBI Official Synonym Full Names: LIM and senescent cell antigen-like domains 1
• NCBI Official Symbol: LIMS1
• NCBI Official Synonym Symbols: PINCH; PINCH1; PINCH-1
• NCBI Protein Information: LIM and senescent cell antigen-like-containing domain protein 1
• UniProt Protein Name: LIM and senescent cell antigen-like-containing domain protein 1
• Protein Family: LIM and senescent cell antigen-like-containing domain protein
• UniProt Gene Name: LIMS1
• UniProt Synonym Gene Names: PINCH; PINCH1; PINCH-1
• UniProt Entry Name: LIMS1_HUMAN

NCBI Description

The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

LIMS1: Effector of integrin and growth factor signaling, coupling surface receptors to downstream signaling molecules involved in the regulation of cell survival, cell proliferation and cell differentiation. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q12.3

Cellular Component: protein complex; focal adhesion; perinuclear region of cytoplasm; plasma membrane; intercellular junction; cytosol

Molecular Function: protein binding; zinc ion binding; protein kinase binding

Biological Process: intercellular junction assembly and maintenance; chordate embryonic development; protein heterooligomerization; cell-matrix adhesion; establishment and/or maintenance of cell polarity; cell aging; regulation of epithelial cell proliferation; establishment of protein localization; cell-cell adhesion; positive regulation of focal adhesion formation; epithelial to mesenchymal transition; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; positive regulation of GTPase activity