Western Blot (WB)
(Mouse Aak1 Antibody (Center) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the Aak1 antibody detected the Aak1 protein (arrow).)
Rabbit Aak1 Polyclonal Antibody | anti-Aak1 antibody
Mouse Aak1 Antibody (Center)
Gene Names
Aak1; C79663; R75501; AU067724; AU067726; BC028270; mKIAA1048; 9630042K20; D6Ertd245e; 5530400K14Rik
Reactivity
Mouse (Predicted Reactivity: Bovine, Human, Pig, Rat)
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Aak1; Polyclonal Antibody; Mouse Aak1 Antibody (Center); AP2-associated protein kinase 1; Adaptor-associated kinase 1; Kiaa1048; anti-Aak1 antibody
Host
Rabbit
Reactivity
Mouse (Predicted Reactivity: Bovine, Human, Pig, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Aak1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 279-306 amino acids from the Central region of mouse Aak1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
279-306
Sequence Length
878
Applicable Applications for anti-Aak1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
MOUSE
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Mouse Aak1 Antibody (Center) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the Aak1 antibody detected the Aak1 protein (arrow).)
Western Blot (WB)
(Mouse Aak1 Antibody (Center) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the Aak1 antibody detected the Aak1 protein (arrow).)
Immunohistochemistry (IHC)
(Mouse Aak1 Antibody (Center) (MBS9214771)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Aak1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Mouse Aak1 Antibody (Center) (MBS9214771)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Aak1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-Aak1 antibody
Aak1 phosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis (By similarity).
Product Categories/Family for anti-Aak1 antibody
References
Munton, R.P., et al. Mol. Cell Proteomics 6(2):283-293(2007)
Nishimura, M., et al. DNA Res. 11(5):315-323(2004)
Jha, A., et al. J. Biol. Chem. 279(3):2281-2290(2004)
Zambrowicz, B.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(24):14109-14114(2003)
Hansen, J., et al. Proc. Natl. Acad. Sci. U.S.A. 100(17):9918-9922(2003)
Nishimura, M., et al. DNA Res. 11(5):315-323(2004)
Jha, A., et al. J. Biol. Chem. 279(3):2281-2290(2004)
Zambrowicz, B.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(24):14109-14114(2003)
Hansen, J., et al. Proc. Natl. Acad. Sci. U.S.A. 100(17):9918-9922(2003)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
103346
NCBI Official Full Name
AP2 associated kinase 1 isoform 1
NCBI Official Synonym Full Names
AP2 associated kinase 1
NCBI Official Symbol
Aak1
NCBI Official Synonym Symbols
C79663; R75501; AU067724; AU067726; BC028270; mKIAA1048; 9630042K20; D6Ertd245e; 5530400K14Rik
NCBI Protein Information
AP2 associated kinase 1
UniProt Protein Name
AP2-associated protein kinase 1
Protein Family
UniProt Gene Name
Aak1
UniProt Synonym Gene Names
Kiaa1048
UniProt Entry Name
AAK1_MOUSE
Uniprot Description
AAK1: an NAK kinase. Plays a regulatory role in clathrin-mediated endocytosis, including the regulation of fatty acid synthesis and cholesterol synthesis.
Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Other group; NAK family
Cellular Component: membrane; plasma membrane; coated pit
Molecular Function: transferase activity; protein serine/threonine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity
Biological Process: endocytosis; phosphorylation; protein amino acid phosphorylation
Similar Products
Product Notes
The Aak1 aak1 (Catalog #AAA9214771) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 279-306. The Mouse Aak1 Antibody (Center) reacts with Mouse (Predicted Reactivity: Bovine, Human, Pig, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Aak1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the Aak1 aak1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aak1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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