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Western Blot (WB)
(Western blot analysis of PI 3 Kinase p85 beta on different lysates using anti-PI 3 Kinase p85 beta antibody at 1/1, 000 dilution. Positive control: Lane 1: Raji Lane 2: Hela Lane 3: MCF-7 Lane 4: U937)
Rabbit anti-Human, Rat PI3 Kinase p85 beta Monoclonal Antibody | anti-PIK3R2 antibody
PI 3 Kinase p85 beta Antibody
Gene Names
PIK3R2; p85; MPPH; P85B; p85-BETA
Reactivity
Human, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
PI3 Kinase p85 beta; Monoclonal Antibody; PI 3 Kinase p85 beta Antibody; p85 antibody; p85 beta antibody; P85B antibody; P85B_HUMAN antibody; Phosphatidylinositol 3 kinase antibody; Phosphatidylinositol 3 kinase regulatory beta subunit antibody; Phosphatidylinositol 3 kinase regulatory subunit beta antibody; Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2 antibody; Phosphatidylinositol 3 kinase; regulatory subunit; polypeptide 2 (p85 beta) antibody; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta antibody; Phosphatidylinositol 3-kinase regulatory subunit beta antibody; Phosphoinositide 3 kinase regulatory subunit 2 (beta) antibody; Phosphoinositide 3 kinase regulatory subunit 2 antibody; Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta) antibody; Phosphoinositide 3 kinase regulatory subunit polypeptide 2 antibody; Phosphoinositide 3 kinase; regulatory subunit 2 (beta) antibody; regulatory subunit 2 (p85 beta) antibody; PI3 kinase p85 beta subunit antibody; PI3 kinase p85 subunit beta antibody; PI3-kinase regulatory subunit beta antibody; PI3-kinase subunit p85-beta antibody; PI3K antibody; PI3K regulatory subunit beta antibody; PIK3R 2 antibody; PIK3R2 antibody; PtdIns 3 kinase p85 beta antibody; PtdIns-3-kinase regulatory subunit beta antibody; PtdIns-3-kinase regulatory subunit p85-beta antibody; anti-PIK3R2 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Monoclonal
Clone Number
ST04-77
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PIK3R2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western blot analysis of PI 3 Kinase p85 beta on different lysates using anti-PI 3 Kinase p85 beta antibody at 1/1, 000 dilution. Positive control: Lane 1: Raji Lane 2: Hela Lane 3: MCF-7 Lane 4: U937)
Western Blot (WB)
(Western blot analysis of PI 3 Kinase p85 beta on different lysates using anti-PI 3 Kinase p85 beta antibody at 1/1, 000 dilution. Positive control: Lane 1: Raji Lane 2: Hela Lane 3: MCF-7 Lane 4: U937)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PI 3 Kinase p85 beta antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PI 3 Kinase p85 beta antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat colon tissue using anti-PI 3 Kinase p85 beta antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat colon tissue using anti-PI 3 Kinase p85 beta antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PI 3 Kinase p85 beta antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PI 3 Kinase p85 beta antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining PI 3 Kinase p85 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining PI 3 Kinase p85 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining PI 3 Kinase p85 beta in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining PI 3 Kinase p85 beta in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells with PI 3 Kinase p85 beta antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells with PI 3 Kinase p85 beta antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-PIK3R2 antibody
Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85alpha and p85beta ), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110alpha and p110beta interact with p85alpha, and p110alpha has also been shown to interact with p85beta in vitro. p110alpha expression is restricted to white blood cells. It has been shown to bind p85alpha and p85beta , but it apparently does not phosphorylate these subunits. p110alpha seems to have the capacity to autophosphorylate. p110alpha does not interact with the p85 subunits. It has been shown to be activated by alpha and beta heterotrimeric G proteins.
Product Categories/Family for anti-PIK3R2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
728
NCBI Official Full Name
phosphatidylinositol 3-kinase regulatory subunit beta
NCBI Official Synonym Full Names
phosphoinositide-3-kinase, regulatory subunit 2 (beta)
NCBI Official Symbol
PIK3R2
NCBI Official Synonym Symbols
p85; MPPH; P85B; p85-BETA
NCBI Protein Information
phosphatidylinositol 3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta; PI3K regulatory subunit beta; ptdIns-3-kinase regulatory subunit p85-beta; phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; phosphatidylinositol 3-kinase, reg
UniProt Protein Name
Phosphatidylinositol 3-kinase regulatory subunit beta
Protein Family
UniProt Gene Name
PIK3R2
UniProt Synonym Gene Names
PI3-kinase regulatory subunit beta; PI3K regulatory subunit beta; PtdIns-3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta
UniProt Entry Name
P85B_HUMAN
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Product Notes
The PIK3R2 pik3r2 (Catalog #AAA9435680) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PI 3 Kinase p85 beta Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PI3 Kinase p85 beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the PIK3R2 pik3r2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI3 Kinase p85 beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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