LIF, Recombinant, Human Recombinant Protein | LIF recombinant protein
LIF, Recombinant, Human (Leukemia Inhibitory Factor, Differentiation-stimulating Factor, D Factor, Melanoma-derived LPL Inhibitor, MLPLI, Emfilermin, HILDA)
Gene Names
LIF; CDF; DIA; HILDA
Purity
Affinity Purified
Synonyms
LIF; Recombinant; Human; Human (Leukemia Inhibitory Factor; Differentiation-stimulating Factor; D Factor; Melanoma-derived LPL Inhibitor; MLPLI; Emfilermin; HILDA); Leukemia Inhibitory Factor; HILDA; LIF recombinant protein
Host
E Coli
Purity/Purification
Affinity Purified
Form/Format
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 20% glycerol, 50mM sodium chloride.
Concentration
~1mg/ml (varies by lot)
Sequence
MHHHHHHMSP ILGYWKIKGL VQPTRLLLEY LEEKYEEHLY ERDEGDKWRN KKFELGLEFP NLPYYIDGDV KLTQSMAIIR YIADKHNMLG GCPKERAEIS MLEGAVLDIR YGVSRIAYSK DFETLKVDFL SKLPEMLKMF EDRLCHKTYL NGDHVTHPDF MLYDALDVVL YMDPMCLDAF PKLVCFKKRI EAIPQIDKYL KSSKYIAWPL QGWQATFGGG DHPPKSDLVP RGSHMSPLPI TPVNATCAIR HPCHNNLMNQ IRSQLAQLNG SANALFILYY TAQGEPFPNN LDKLCGPNVT DFPPFHANGT EKAKLVELYR IVVYLGTSLG NITRDQKILN PSALSLHSKL NATADILRGL LSNVLCRLCS KYHVGHVDVT YGPDTSGKDV FQKKKLGCQL LGKYKQIIAV LAQAF
Source Information
Recombinant corresponding to aa23-202 of human LIF protein, fused to His GST-tag at N-terminus, expressed in E.coli.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Temperature: Blue Ice
Shipping Temperature: Blue Ice
SDS-Page
(15% SDS-PAGE using 3ug of L2040-11A.)
Related Product Information for LIF recombinant protein
Leukemia inhibitory factor, also known as LIF, is a pleiotropic cytokine that is expressed by a wide variety of cells including activated T lymphocytes, monocytes, mast cells and neuronal cells. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface.
Product Categories/Family for LIF recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47.2kD (415aa) confirmed by MALDI-TOF
NCBI Official Full Name
leukemia inhibitory factor
NCBI Official Synonym Full Names
leukemia inhibitory factor (cholinergic differentiation factor)
NCBI Official Symbol
LIF
NCBI Official Synonym Symbols
CDF; DIA; HILDA
NCBI Protein Information
leukemia inhibitory factor; MLPLI; D factor; OTTHUMP00000198698; human interleukin in DA cells; melanoma-derived LPL inhibitor; differentiation-inducing factor; hepatocyte-stimulating factor III; cholinergic differentiation factor; differentiation stimulating factor; differentiation-stimulating factor; differentiation inhibitory activity
UniProt Protein Name
Leukemia inhibitory factor
Protein Family
UniProt Gene Name
LIF
UniProt Synonym Gene Names
HILDA
UniProt Entry Name
LIF_HUMAN
