Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with Anti Human/Mouse CD45R (PE) and 0.06 ug of Mouse IgG1 kappa Isotype Control (biotin) (left) or 0.06 ug of Anti-Phycoerythrin (biotin) (right) followed by Streptavidin (APC). Total viable cells were used for analysis.)
Mouse Phycoerythrin Monoclonal Antibody
Phycoerythrin antibody (biotin)
Applications
Flow Cytometry, Functional Assay
Synonyms
Phycoerythrin; Monoclonal Antibody; Phycoerythrin antibody (biotin); Monoclonal Phycoerythrin; Anti-Phycoerythrin; PE; Anti Phycoerythrin; anti-Phycoerythrin antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
PE-DLF
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Applicable Applications for anti-Phycoerythrin antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 0.125 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Biological Significance
The PE-DLF monoclonal antibody reacts with phycoerythrin, PE-Cy5 and PE-Cy7, with no crossreactivity to fluorescein or allophycocyanin. When using PE-DLF as a second-step antibody, in combination with an appropriate third step, to bind a PE-conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated PE antibody. Therefore no interference or quenching of the PE fluorochrome is observed. The PE-DLF can be used for separation of cells labeled with PE-conjugated antibodies or staining. Please note that the optimal concentration for this antibody will vary depending on the density of the primary PE-conjugated antibody and the application tested.
Tag/Conjugate
Biotin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with Anti Human/Mouse CD45R (PE) and 0.06 ug of Mouse IgG1 kappa Isotype Control (biotin) (left) or 0.06 ug of Anti-Phycoerythrin (biotin) (right) followed by Streptavidin (APC). Total viable cells were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of C57BL/6 splenocytes with Anti Human/Mouse CD45R (PE) and 0.06 ug of Mouse IgG1 kappa Isotype Control (biotin) (left) or 0.06 ug of Anti-Phycoerythrin (biotin) (right) followed by Streptavidin (APC). Total viable cells were used for analysis.)
Related Product Information for anti-Phycoerythrin antibody
Mouse monoclonal Phycoerythrin antibody (biotin)
Product Categories/Family for anti-Phycoerythrin antibody
Similar Products
Product Notes
The Phycoerythrin (Catalog #AAA834790) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Phycoerythrin can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 0.125 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the Phycoerythrin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phycoerythrin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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