Flow Cytometry (FC/FACS)
(Staining of C57Bl/6 bone marrow cells with CD45R antibody and 0.25 ug of Rat IgG2a kappa Isotype Control (FITC) (left) or 0.25 ug of Anti-Mouse IgM (FITC) (right). Total viable cells were used for analysis.)
Rat anti-Mouse IgM antibody (FITC) Monoclonal Antibody | anti-IgM antibody
Rat anti-Mouse IgM antibody (FITC)
Applications
Flow Cytometry, Functional Assay
Synonyms
Rat anti-Mouse IgM antibody (FITC); Monoclonal Antibody; Monoclonal anti-IgM; Anti-anti-IgM; Immunoglobulin M; Anti IgM; Ig M; Anti Anti IgM; anti-IgM antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
121-15F9
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Applicable Applications for anti-IgM antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 0.5 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Biological Significance
The 121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated 121-15F9 can be used as a detection secondary for mouse IgM.
Tag/Conjugate
FITC
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of C57Bl/6 bone marrow cells with CD45R antibody and 0.25 ug of Rat IgG2a kappa Isotype Control (FITC) (left) or 0.25 ug of Anti-Mouse IgM (FITC) (right). Total viable cells were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of C57Bl/6 bone marrow cells with CD45R antibody and 0.25 ug of Rat IgG2a kappa Isotype Control (FITC) (left) or 0.25 ug of Anti-Mouse IgM (FITC) (right). Total viable cells were used for analysis.)
Related Product Information for anti-IgM antibody
Monoclonal Rat anti-Mouse IgM antibody (FITC)
Product Categories/Family for anti-IgM antibody
Similar Products
Product Notes
The IgM (Catalog #AAA834534) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Rat anti-Mouse IgM antibody (FITC) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 0.5 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the IgM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rat anti-Mouse IgM antibody (FITC), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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