Mouse Phosphotyrosine, mixed clones Monoclonal Antibody
Phosphotyrosine, mixed clones
Applications
Western Blot, Immunoprecipitation
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Phosphotyrosine; mixed clones; Monoclonal Antibody; Anti -Phosphotyrosine; anti-Phosphotyrosine; mixed clones antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3G244
Specificity
PY-Plus reacts specifically with tyrosine-phosphorylated proteins. It shows no crossreactivity to phosphoserine or phosphothreonine. On Western blots, specific inhibition of each individual antibody and the cocktail reactivity can be achieved by pre-incubation of the antibody with 20 mM phosphotyrosine, whereas pre-incubation with 20 mM phosphoserine or 20 mM phosphothreonine is ineffective.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in phosphate buffered saline, pH 7.4, 40% glycerol and 0.1% proclin.
Applicable Applications for anti-Phosphotyrosine, mixed clones antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Western Blotting, Immunoprecipitation.
Dilution: Western Blotting: 1:500 to 1:1,000
Immunoprecipitation:
Dilution: Western Blotting: 1:500 to 1:1,000
Immunoprecipitation:
Immunogen
Tyrosine-phosphorylated proteins
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Phosphotyrosine, mixed clones antibody
Protein tyrosine phosphorylation plays a central role in the transmission of signals from the cell surface to the nucleus.(3,7) Reversible phosphorylation of cellular proteins on specific tyrosine residues is involved in a variety of fundamental cellular processes, including survival, proliferation, differentiation, and transformation.(3,6,7) The use of antibodies which specifically recognize phosphotyrosine is widely accepted as one of the most powerful tools for detection, characterization, and purification of proteins that are either constitutively or inducibly phosphorylated on tyrosine (where even a single phosphorylated tyrosine residue is sufficient for detection).(1,2,4,5)
Product Categories/Family for anti-Phosphotyrosine, mixed clones antibody
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Product Notes
The Phosphotyrosine, mixed clones (Catalog #AAA602818) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Phosphotyrosine, mixed clones can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blotting, Immunoprecipitation. Dilution: Western Blotting: 1:500 to 1:1,000 Immunoprecipitation:. Researchers should empirically determine the suitability of the Phosphotyrosine, mixed clones for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosphotyrosine, mixed clones, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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