Western Blot (WB)
(HMGB4 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HMGB4 antibody detected the HMGB4 protein (arrow).)
Rabbit anti-Human HMGB4 Polyclonal Antibody | anti-Hmgb4 antibody
HMGB4, ID (HMGB4, High mobility group protein B4)
Gene Names
Hmgb4; AI326135; 1700001F22Rik; RP23-284L15.1
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
HMGB4; Polyclonal Antibody; ID (HMGB4; High mobility group protein B4); Anti -HMGB4; anti-Hmgb4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-Hmgb4 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
HMGB4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-73 amino acids from the Central region of human HMGB4.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(HMGB4 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HMGB4 antibody detected the HMGB4 protein (arrow).)
Western Blot (WB)
(HMGB4 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HMGB4 antibody detected the HMGB4 protein (arrow).)
Related Product Information for anti-Hmgb4 antibody
High Mobility Group (HMG) proteins, a group of chromosomal proteins common to all eukaryotes, bind DNA in a non-sequence-specific fashion to promote chromatin function and gene regulation, helping with transcription, replication, recombination, and DNA repair. HMGB4 is HMG2-like, isoform 2, high-mobility group box 4. This gene can be found on Chromosome 1. HMGB4 contains two HMG-box regions, and is found in a variety of eukaryotic chromosomal proteins. HMGB proteins are phosphorylated to various extents. The existence of differentially modified forms increases the number of distinct HMGB protein variants in plant chromatin that may be adapted to certain functions.
Product Categories/Family for anti-Hmgb4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,604 Da
NCBI Official Full Name
high mobility group protein B4
NCBI Official Synonym Full Names
high-mobility group box 4
NCBI Official Symbol
Hmgb4
NCBI Official Synonym Symbols
AI326135; 1700001F22Rik; RP23-284L15.1
NCBI Protein Information
high mobility group protein B4
UniProt Protein Name
High mobility group protein B4
Protein Family
UniProt Gene Name
Hmgb4
UniProt Entry Name
HMGB4_MOUSE
Uniprot Description
HMGB4: Belongs to the HMGB family.
Cellular Component: chromosome; nucleus
Molecular Function: DNA binding; chromatin binding
Biological Process: chromatin remodeling; regulation of transcription, DNA-dependent
Research Articles on Hmgb4
Similar Products
Product Notes
The Hmgb4 hmgb4 (Catalog #AAA6001377) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HMGB4, ID (HMGB4, High mobility group protein B4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HMGB4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the Hmgb4 hmgb4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HMGB4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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