Mouse Phosphatase 1A Alpha Monoclonal Antibody | anti-PPM1A antibody

Mouse Anti Human Protein Phosphatase 1A Alpha Isoform

Cat. Num. AAA140200

• Gene Names: PPM1A; PP2CA; PP2Calpha; PP2C-ALPHA
• Applications: ELISA, Western Blot
• Purity: PPM1A antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Phosphatase 1A Alpha; Monoclonal Antibody; Mouse Anti Human Protein Phosphatase 1A Alpha Isoform; PPM1A Antibody; Protein Phosphatase 1A Alpha Isoform; Mouse Anti Human; Protein phosphatase 1A; EC 3.1.3.16; Protein phosphatase 2C isoform alpha; PP2C-alpha; IA; PPM1A; PP2CA; MGC9201; anti-PPM1A antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b heavy chain and kappa light chain
• Clone Number: Pp6c7AT
• Purity/Purification: PPM1A antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Sequence Length: 455

• Applicable Applications for anti-PPM1A antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: PPM1A antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:250 ~ 500. Recommended starting dilution is 1:250.
• Immunogen: Anti-human PPM1A mAb, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PPM1A amino acids 1-382 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-PPM1A antibody

Introduction: Protein Phosphatase 2C alpha is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding two distinct isoforms have been described. Protein phosphatase 2C(PP2Calpha) is a Mn2+- or Mg2+-dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes.

Product Categories/Family for anti-PPM1A antibody

Monoclonal Antibodies; Anti-Human Enzymes; Monoclonal Antibodies; Anti Human Enzyme

NCBI and Uniprot Product Information

• NCBI GI #: 193211600
• NCBI GeneID: 5494
• NCBI Accession #: NP_808821.2
• NCBI GenBank Nucleotide #: NM_177952.2
• Molecular Weight: 51,366 Da
• NCBI Official Full Name: protein phosphatase 1A isoform 3
• NCBI Official Synonym Full Names: protein phosphatase, Mg2+/Mn2+ dependent, 1A
• NCBI Official Symbol: PPM1A
• NCBI Official Synonym Symbols: PP2CA; PP2Calpha; PP2C-ALPHA
• NCBI Protein Information: protein phosphatase 1A; protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
• UniProt Protein Name: Protein phosphatase 1A
• Protein Family: Protein phosphatase
• UniProt Gene Name: PPM1A
• UniProt Synonym Gene Names: PPPM1A; PP2C-alpha
• UniProt Entry Name: PPM1A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

PPPM1A: a Ser/Thr protein phosphatase of the PP2C family. Inhibits the activation of p38 and JNK kinase cascades induced by environmental stresses. Can also dephosphorylate cyclin-dependent kinases and may play a role in cell cycle control. Overexpression of this phosphatase activates the expression of p53, leading to G2/M cell cycle arrest and apoptosis.

Protein type: EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: nucleoplasm; neuron projection; membrane; voltage-gated calcium channel complex; cytosol; nucleus

Molecular Function: protein C-terminus binding; protein binding; signal transducer activity; calmodulin-dependent protein phosphatase activity; manganese ion binding; magnesium ion binding; protein serine/threonine phosphatase activity

Biological Process: transcription initiation from RNA polymerase II promoter; N-terminal protein myristoylation; Wnt receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription from RNA polymerase II promoter; negative regulation of NF-kappaB import into nucleus; protein amino acid dephosphorylation; dephosphorylation; transforming growth factor beta receptor signaling pathway; insulin receptor signaling pathway; gene expression; negative regulation of transforming growth factor beta receptor signaling pathway; cell cycle arrest; positive regulation of Wnt receptor signaling pathway