Western Blot (WB)
(Western Blot analysis of Human heart tissue using Prohibitin Monoclonal Antibody at dilution of 1:1000.)
Mouse anti-Human, Rat PHB Monoclonal Antibody | anti-PHB antibody
PHB Monoclonal Antibody
Gene Names
PHB; PHB1; HEL-215; HEL-S-54e
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Caprylic acid/ammonium sulfate precipitation
Synonyms
PHB; Monoclonal Antibody; PHB Monoclonal Antibody; Epididymis luminal protein 215; Epididymis secretory sperm binding protein Li 54e; HEL 215; HEL S 54e; PHB1; Prohibitin; anti-PHB antibody
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
73
Purity/Purification
Caprylic acid/ammonium sulfate precipitation
Concentration
1mg/mL (varies by lot)
Sequence Length
272
Applicable Applications for anti-PHB antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
IHC: 1:20-1:200
IF: 1:10-1:100
Immunogen
Fusion protein of Prohibitin
Buffer
PBS with 0.1% sodium azide, 50% glycerol, PH7.3
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western Blot analysis of Human heart tissue using Prohibitin Monoclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of Human heart tissue using Prohibitin Monoclonal Antibody at dilution of 1:1000.)
Related Product Information for anti-PHB antibody
Prohibitin, also known as PHB, a member of the Band-7 family of proteins, is widely distributed in bacteria, plants, yeast, protozoa and mammals and is important in cell proliferation, differentiation and apoptosis. This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription. Recent study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis.
Product Categories/Family for anti-PHB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 30kDa
Observed: 30kDa
Observed: 30kDa
NCBI Official Full Name
prohibitin isoform 1
NCBI Official Synonym Full Names
prohibitin
NCBI Official Symbol
PHB
NCBI Official Synonym Symbols
PHB1; HEL-215; HEL-S-54e
NCBI Protein Information
prohibitin
UniProt Protein Name
Prohibitin
Protein Family
UniProt Gene Name
PHB
NCBI Description
This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Uniprot Description
Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
Research Articles on PHB
Similar Products
Product Notes
The PHB phb (Catalog #AAA2540232) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PHB Monoclonal Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PHB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:20-1:200 IF: 1:10-1:100. Researchers should empirically determine the suitability of the PHB phb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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