Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PHB antibody.)
Rabbit ERK1/P44 Antibody | anti-ERK1/P44 antibody
ERK1/P44 Antibody
Gene Names
PHB; PHB1; HEL-215; HEL-S-54e
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Synonyms
ERK1/P44; ERK1/P44 Antibody; PHB; PHB1; anti-ERK1/P44 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
272
Applicable Applications for anti-ERK1/P44 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Recombinant protein of human PHB
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PHB antibody.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PHB antibody.)
Related Product Information for anti-ERK1/P44 antibody
The prohibitins, called PHB1 and PHB2, are highly conserved proteins that are present in multiple compartments in eukaryotic cells. PHB1 is 30kDa tumor suppressor protein involved in cell cycle control (1). PHB1 has been found in mitochondria, the nucleus and the plasma membrane, as well as extracellularly in circulation (2). In mitochondria prohibitins mainly exist as membrane-bound ring complexes and function as chaperones maintaining mitochondrial protein stability during protein synthesis and transportation (3, 4). In the nucleus prohibitins interact with transcription factors such as Rb and p53 to regulate target gene transcription (2, 5). Extracellular prohibitin can bind and activate C3 to enhance complement activation (6).
Product Categories/Family for anti-ERK1/P44 antibody
References
1. McClung, J.K. et al. (1995) Exp Gerontol 30, 99-124. 2. Mishra, S. et al. (2006) J. Cell. Mol. Med. 10, 353-363. 3. Nijtmans, L.G. et al. (2000) EMBO J. 19, 2444-2451. 4. Tatsuta, T. et al. (2005) Mol. Biol. Cell 16, 248-259. 5. Fusaro, G. et al. (2003) J. Biol. Chem. 278, 47853-47861. 6. Mishra, S. et al. (2007) Mol. Immunol. 44, 1907-1912.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30kDa
NCBI Official Full Name
prohibitin isoform 1
NCBI Official Synonym Full Names
prohibitin
NCBI Official Symbol
PHB
NCBI Official Synonym Symbols
PHB1; HEL-215; HEL-S-54e
NCBI Protein Information
prohibitin
UniProt Protein Name
Prohibitin
UniProt Gene Name
PHB
NCBI Description
This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Uniprot Description
Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
Research Articles on ERK1/P44
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Product Notes
The ERK1/P44 phb (Catalog #AAA856882) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ERK1/P44 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ERK1/P44 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the ERK1/P44 phb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERK1/P44, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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