Western Blot (WB)
(Western blot analysis of TGM4 Antibody (Center) in CEM cell line lysates (35ug/lane). TGM4 (arrow) was detected using the purified Pab.)
Rabbit anti-Human TGM4 Polyclonal Antibody | anti-TGM4 antibody
TGM4, ID (Protein-glutamine gamma-glutamyltransferase 4, Transglutaminase-4, TGase-4, Prostate Transglutaminase, Transglutaminase P, TGase P, TGP, TG(P), Prostate-specific Transglutaminase, Fibrinoligase) (PE)
Gene Names
TGM4; TGP; hTGP
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Immunoaffinity chromatography
Synonyms
TGM4; Polyclonal Antibody; ID (Protein-glutamine gamma-glutamyltransferase 4; Transglutaminase-4; TGase-4; Prostate Transglutaminase; Transglutaminase P; TGase P; TGP; TG(P); Prostate-specific Transglutaminase; Fibrinoligase) (PE); anti-TGM4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human TGM4
Purity/Purification
Purified by Immunoaffinity chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-TGM4 antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-1:50
FLISA: 1:1,000
IHC: 1:50-1:100
WB: 1:100-1:500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:50-1:100
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide between a portion within aa142-172 located in the center region of human TGM4 (conjugated to KLH)
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of TGM4 Antibody (Center) in CEM cell line lysates (35ug/lane). TGM4 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of TGM4 Antibody (Center) in CEM cell line lysates (35ug/lane). TGM4 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostate carcinoma with TGM4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostate carcinoma with TGM4 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of CEM cells using TGM4 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of CEM cells using TGM4 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-TGM4 antibody
References
1. Jiang, W.G., et.al., Microvasc. Res. 77(2), 150-157 (2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
protein-glutamine gamma-glutamyltransferase 4
NCBI Official Synonym Full Names
transglutaminase 4
NCBI Official Symbol
TGM4
NCBI Official Synonym Symbols
TGP; hTGP
NCBI Protein Information
protein-glutamine gamma-glutamyltransferase 4; TG(P); TGase P; TGase-4; fibrinoligase; transglutaminase P; transglutaminase-4; prostate transglutaminase; transglutaminase 4 (prostate); prostate-specific transglutaminase
UniProt Protein Name
Protein-glutamine gamma-glutamyltransferase 4
Protein Family
UniProt Gene Name
TGM4
UniProt Synonym Gene Names
TG(P); TGP; TGase P; TGase-4
UniProt Entry Name
TGM4_HUMAN
Uniprot Description
TGM4: Associated with the mammalian reproductive process. Catalyzes the cross-linking of proteins and the conjugation of polyamines to specific proteins in the seminal tract. Belongs to the transglutaminase superfamily. Transglutaminase family.
Protein type: Transferase; EC 2.3.2.13
Chromosomal Location of Human Ortholog: 3p22-p21.33
Cellular Component: extracellular matrix; Golgi apparatus; cytoplasm
Molecular Function: protein-glutamine gamma-glutamyltransferase activity; metal ion binding
Biological Process: peptide cross-linking; mating plug formation
Research Articles on TGM4
Similar Products
Product Notes
The TGM4 tgm4 (Catalog #AAA6503453) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TGM4, ID (Protein-glutamine gamma-glutamyltransferase 4, Transglutaminase-4, TGase-4, Prostate Transglutaminase, Transglutaminase P, TGase P, TGP, TG(P), Prostate-specific Transglutaminase, Fibrinoligase) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TGM4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-1:50 FLISA: 1:1,000 IHC: 1:50-1:100 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TGM4 tgm4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TGM4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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