Rabbit PI3 kinase Polyclonal Antibody | anti-PI3 kinase antibody

PI3 kinase Antibody

Cat. Num. AAA841179

• Gene Names: Pik3ca; p110; caPI3K; p110alpha; 6330412C24Rik
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: PI3 kinase; Polyclonal Antibody; PI3 kinase Antibody; PIK3CA; MGC142161; MGC142163; PI3K; anti-PI3 kinase antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: 100 ug (0.5 mg/ml) affinity purified rabbit anti-P13 kinase polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.; Appearance: Colorless liquid
• Concentration: 0.5mg/ml (varies by lot)
• Sequence Length: 1068

• Applicable Applications for anti-PI3 kinase antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: The antibody can be used for Western blot analysis (1-4 ug/ml) and Immunohistochemistry (5 ug/ml). However, the optimal conditions should be determined individually. Blocking peptide is available separately.
• Immunogen: Synthetic peptide surrounding amino acid 505 of mouse P13 kinase.
• Positive Control: Western Blot: Jurkat cell lysate. IHC: colon tissue
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-PI3 kinase antibody

Background: Phosphatidylinositol 3-kinase (PI 3-kinase), is a phosphoinositide-specific protein kinase that plays a central role in mitogenic signal transduction leading to cellular regulation, cell growth and differentiation, and oncogenesis. PI 3-kinase associates with, and is activated by a wide range of tyrosine kinase receptors including EGF, PDGF, CSF-1, erbB-3 and insulin receptor, non-receptor protein-tyrosine kinases of the src family (e.g. pp60src), and crk and abl proto-oncogene products. PI 3-kinase is involved in signal transduction in terminally differentiated, activated cells like platelets and neutrophils.

Product Categories/Family for anti-PI3 kinase antibody

Antibodies & Supporting Tools; Primary Antibodies (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 254540080
• NCBI GeneID: 18706
• NCBI Accession #: NP_032865
• NCBI GenBank Nucleotide #: NM_008839.2
• Molecular Weight: 124,412 Da
• NCBI Official Full Name: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• NCBI Official Synonym Full Names: phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
• NCBI Official Symbol: Pik3ca
• NCBI Official Synonym Symbols: p110; caPI3K; p110alpha; 6330412C24Rik
• NCBI Protein Information: phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• UniProt Protein Name: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• UniProt Gene Name: Pik3ca
• UniProt Synonym Gene Names: PI3-kinase subunit alpha; PI3K-alpha; PI3Kalpha; PtdIns-3-kinase subunit alpha; PtdIns-3-kinase subunit p110-alpha; p110alpha
• UniProt Entry Name: PK3CA_MOUSE

Uniprot Description

PIK3CA: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4- phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5- bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1- AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS. Heterodimer of a catalytic subunit PIK3CA and a p85 regulatory subunit (PIK3R1, PIK3R2 or PIK3R3). Interacts with IRS1 in nuclear extracts. Interacts with RUFY3. Interacts with RASD2. Interacts with APPL1. Interacts with HRAS1 and KRAS. Interaction with HRAS1/KRAS is required for PI3K pathway signaling and cell proliferation stimulated by EGF and FGF2. Belongs to the PI3/PI4-kinase family.

Protein type: Kinase, lipid; EC 2.7.1.153; Oncoprotein; Carbohydrate Metabolism - inositol phosphate; Motility/polarity/chemotaxis; EC 2.7.11.1

Cellular Component: lamellipodium; plasma membrane; intracellular; phosphoinositide 3-kinase complex

Molecular Function: phosphatidylinositol-4,5-bisphosphate 3-kinase activity; 1-phosphatidylinositol-3-kinase activity; nucleotide binding; phosphatidylinositol-4-phosphate 3-kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; insulin receptor substrate binding; transferase activity, transferring phosphorus-containing groups; kinase activity; phosphotransferase activity, alcohol group as acceptor; protein kinase activator activity; ATP binding

Biological Process: phosphoinositide-mediated signaling; phosphoinositide phosphorylation; positive regulation of protein kinase activity; protein kinase B signaling cascade; regulation of multicellular organism growth; glucose metabolic process; angiogenesis; negative regulation of neuron apoptosis; phosphorylation; positive regulation of peptidyl-serine phosphorylation; protein amino acid phosphorylation