Mouse anti-Human PDK1 Monoclonal Antibody | anti-PDK1 antibody

PDK1 (PDPK1, PI-P3 Dependent Kinase, 3a Phosphoinositide dependent Protein Kinase 1)

Cat. Num. AAA605315

• Gene Names: PDK1; 3'-phosphoinositide-dependent protein kinase 1; 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1; ATPDK1; PDK1; T32M21.110; T32M21_110
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PDK1; Monoclonal Antibody; PDK1 (PDPK1; PI-P3 Dependent Kinase; 3a Phosphoinositide dependent Protein Kinase 1); Anti -PDK1 (PDPK1; anti-PDK1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 10B2759
• Specificity: Recognizes human PDK1 (PDPK1, PI-P3 Dependent Kinase, 3a Phosphoinositide dependent Protein Kinase 1).
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

• Applicable Applications for anti-PDK1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA, Western Blotting.; Dilution: Western Blotting: 1:250-1:1000
• Immunogen: Recombinant human PDK1 (29-436aa) purified from E. coli
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis|The extracts of rat heart (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDK1 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

Related Product Information for anti-PDK1 antibody

PDK1 (pyruvate dehydrogenase kinase isoform 1) is involved in the regulation of enzymatic activity of mammalian pyruvate dehydrogenase (PDH) that is a part of a mitochondrial multienzyme complex to catalyze the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. PDK1 has been found to serve as an effective therapeutic target for inhibition of glioblastoma growth.

Product Categories/Family for anti-PDK1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 18414583
• NCBI GeneID: 830330
• NCBI Accession #: NP_568138.1
• NCBI GenBank Nucleotide #: NM_120533.4
• UniProt Accession #: Q9XF67; Q9LZ74
• Molecular Weight: 54,711 Da
• NCBI Official Full Name: 3'-phosphoinositide-dependent protein kinase 1
• NCBI Official Symbol: PDK1
• NCBI Official Synonym Symbols: 3'-phosphoinositide-dependent protein kinase 1; 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1; ATPDK1; PDK1; T32M21.110; T32M21_110
• NCBI Protein Information: 3'-phosphoinositide-dependent protein kinase 1
• UniProt Protein Name: 3-phosphoinositide-dependent protein kinase 1
• Protein Family: [Pyruvate dehydrogenase
• UniProt Gene Name: PDK1
• UniProt Entry Name: PDPK1_ARATH

NCBI Description

Encodes 3-phosphoinositide-dependent protein kinase that contains pleckstrin homology domain and binds 3-phosphoinositides. It activates the protein kinase AGC2-1 in a phosphatidic acid dependent manner. Phosphorylates S6K1. Interacts with PID, and transphosphorylation by PDK1 increases PID autophosphorylation.

Uniprot Description

Function: May couple lipid signals to the activation-loop phosphorylation of several protein kinases of the so-called AGC kinase family. Interacts via its pleckstrin homology domain with phosphatidic acid, PtdIns3P and PtdIns(3,4)P2 and to a lesser extent with PtdIns(4,5)P2 and PtdIns4P. May play a general role in signaling processes controlling the pathogen/stress response, polar auxin transport and development. Transphosphorylates the AGC protein kinases OXI1/AGC2-1, PK1/S6K1, PK19/S6K2 and PID resulting in their activation. Ref.1 Ref.7 Ref.9 Ref.11 Ref.12

Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Ref.6

Enzyme regulation: Activated by phosphatidic acid (PA) and in response to the fungal elicitor xylanase. Ref.10

Subunit structure: Interacts with the C-terminal PIF domain of the protein kinases D6PK/AGC1-1, OXI1/AGC2-1 and PID. Ref.7 Ref.11

Subcellular location: Cytoplasm

By similarity. Membrane; Peripheral membrane protein

By similarity. Note: Membrane-associated after cell stimulation

Tissue specificity: Ubiquitous. Ref.7

Domain: The PH domain is responsible for the interaction with the 3-phosphoinositides. The activation loop within the kinase domain is the target of phosphorylation. The PIF-binding region in the kinase domain of PDK1 acts as a docking site, enabling it to interact with and enhance the phosphorylation of substrates containing the PIF motif.

Post-translational modification: Phosphorylation on Thr-211 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Thr-211, leading to its own activation.

Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.Contains 1 PH domain.Contains 1 protein kinase domain.

Sequence caution: The sequence CAB85557.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.