PDK1 recombinant protein

PDK1, 29-436aa, Human, His tag, E Coli

Cat. Num. AAA203261

• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: PDK1; 29-436aa; Human; His tag; E Coli; Pyruvate dehydrogenase kinase isozyme 1; Pyruvate dehydrogenase kinase; isozyme 1; isozyme 1 HGNC:8809; Mitochondrial pyruvate dehydrogenase kinase isoenzyme1; Pyruvate dehydrogenase kinase isoform 1; isoenzyme 1.; PDK1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 7.0) containing 100mM NaCl, 0.5mM DTT, 0.1mM EDTA, 0.1mM PMSF, 1mM MgCl2, 40% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSSDSGSSPA SERGVPGQVD FYARFSPSPL SMKQFLDFGS VNACEKTSFM FLRQELPVRL ANIMKEISLL PDNLLRTPSV QLVQSWYIQS LQELLDFKDK SAEDAKAIYD FTDTVIRIRN RHNDVIPTMA QGVIEYKESF GVDPVTSQNV QYFLDRFYMS RISIRMLLNQ HSLLFGGKGK GSPSHRKHIG SINPNCNVLE VIKDGYENAR RLCDLYYINS PELELEELNA KSPGQPIQVV YVPSHLYHMV FELFKNAMRA TMEHHANRGV YPPIQVHVTL GNEDLTVKMS DRGGGVPLRK IDRLFNYMYS TAPRPRVETS RAVPLAGFGY GLPISRLYAQ YFQGDLKLYS LEGYGTDAVI YIKALSTDSI ERLPVYNKAA WKHYNTNHEA DDWCVPSREP KDMTTFRSA
• Sequence Length: 436

• Applicable Applications for PDK1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PDK1 recombinant protein

PDK1 (pyruvate dehydrogenase kinase isoform 1) is involved in the regulation of enzymatic activity of mammalian pyruvate dehydrogenase (PDH) that is a part of a mitochondrial multienzyme complex to catalyze the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. PDK1 has been found to serve as an effective therapeutic target for inhibition of glioblastoma growth. Recombinant human PDK1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PDK1 recombinant protein

Enzymes & Proteases

References

Gudi R., et al. (1995). J Biol chem. 270 (48) : 28989-94; Xie Z., et al. (2006). BMC Cancer. 6 :77;

NCBI and Uniprot Product Information

• NCBI GI #: 4505689
• NCBI GeneID: 5163
• NCBI Accession #: NP_002601
• NCBI GenBank Nucleotide #: NM_002610.4
• UniProt Accession #: Q15118; Q308M4; B2R6T1; B7Z937; D3DPD8; E9PD65
• Molecular Weight: 48.6 kDa (429 aa)
• NCBI Official Full Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial isoform 2
• NCBI Official Synonym Full Names: pyruvate dehydrogenase kinase 1
• NCBI Official Symbol: PDK1
• NCBI Protein Information: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; pyruvate dehydrogenase (acetyl-transferring) kinase isozyme 1, mitochondrial
• UniProt Protein Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
• Protein Family: [Pyruvate dehydrogenase
• UniProt Gene Name: PDK1
• UniProt Synonym Gene Names: PDHK1; PDH kinase 1

NCBI Description

Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.