Rabbit CSF2 Polyclonal Antibody | anti-CSF2 antibody

CSF2 Polyclonal Antibody

Cat. Num. AAA3006311

• Gene Names: CSF2; CSF; GMCSF
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CSF2; Polyclonal Antibody; CSF2 Polyclonal Antibody; Granulocyte-macrophage colony-stimulating factor; GM-CSF; Colony-stimulating factor; CSF; Molgramostin; Sargramostim; GMCSF; anti-CSF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CSF2 polyclonal antibody detects endogenous levels of CSF2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 144

• Applicable Applications for anti-CSF2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human CSF2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CSF2 polyclonal antibody at 1:500 dilutionLane1:THP-1 whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysate)

Related Product Information for anti-CSF2 antibody

Colonystimulating factors(CSFs)were initiallycharacterized bytheir ability to stimulate in vitro colonyformation by hematopoietic progenitorcellsin semi-solidmedia. Several of these CSFs have been assigned an interleukin number, while three (GM-CSF, G-CSF and M-CSF) have retained their CSF designations. The human granulocyte-macrophage colonystimulating factor(GM-CSF) is a pleiotropic cytokinewith a 17 amino acid signal peptide that is cleaved to produce themature formof 127 amino acids. Thematuremurine GM-CSF protein is 124 amino acids and shares 60 percent homology with the human GM-CSF protein. GM-CSFis a glycoprotein thatcan stimulate the proliferation of hematopoieticcellsincluding granulocytes andmacrophages. It has been shown to promote the phosphorylation ofcPLA2 in human neutrophils. The phosphorylation ofcPLA2was accompanied by an increase in the enzyme activity.

NCBI and Uniprot Product Information

• NCBI GI #: 183364
• NCBI GeneID: 1437
• UniProt Accession #: P04141
• Molecular Weight: ~ 23kDa
• NCBI Official Full Name: GM-CSF
• NCBI Official Synonym Full Names: colony stimulating factor 2
• NCBI Official Symbol: CSF2
• NCBI Official Synonym Symbols: CSF; GMCSF
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• Protein Family: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: CSF2
• UniProt Synonym Gene Names: GMCSF; GM-CSF; CSF
• UniProt Entry Name: CSF2_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]