Mouse anti-Human PCLO Monoclonal Antibody | anti-PCLO antibody

Anti-PCLO PE

Cat. Num. AAA1801478

• Gene Names: PCLO; ACZ; PCH3
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: PCLO; Monoclonal Antibody; Anti-PCLO PE; piccolo presynaptic cytomatrix protein; ACZ; PCH3; piccolo; aczonin; anti-PCLO antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: PCLO-01
• Specificity: The mouse monoclonal antibody PCLO-01 recognizes PCLO (Piccolo), a more than 400 kDa multidomain protein expressed mainly in the presynaptic cytoplasmatic matrix of the neurons.
• Purity/Purification: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
• Concentration: 0.1 mg/ml (varies by lot)
• Sequence Length: 5142

• Applicable Applications for anti-PCLO antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: QC tested
• Immunogen: Human recombinant PCLO protein
• Conjugation: PE
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (intracellular staining) of PCLO in human peripheral blood using mouse monoclonal (PCLO-01) PE.)

Related Product Information for anti-PCLO antibody

PCLO (piccolo, also known as aczonin) is a large (more than 400 kDa) multidomain protein of the presynaptic cytomatrix in neurons, that is present in all vertebrate synapses, but is absent from invertebrates. It contains zinc finger and coiled-coil sequences, as well as N-terminal glutamine-rich sequence, and C-terminal PDZ domain followed by two C2 domains (C2A and C2B). In vitro binding and transfection experiments suggested that PCLO binds to multiple proteins including profilin and L-type calcium channels. It is involved in neurotransmitter release and insulin secretion.

NCBI and Uniprot Product Information

• NCBI GI #: 150378539
• NCBI GeneID: 27445
• NCBI Accession #: NP_149015.2
• NCBI GenBank Nucleotide #: NP_149015.2
• UniProt Accession #: Q9Y6V0
• NCBI Official Full Name: protein piccolo isoform 1
• NCBI Official Synonym Full Names: piccolo presynaptic cytomatrix protein
• NCBI Official Symbol: PCLO
• NCBI Official Synonym Symbols: ACZ; PCH3
• NCBI Protein Information: protein piccolo
• UniProt Protein Name: Protein piccolo
• Protein Family: Protein piccolo
• UniProt Gene Name: PCLO
• UniProt Synonym Gene Names: ACZ; KIAA0559
• UniProt Entry Name: PCLO_HUMAN

NCBI Description

The protein encoded by this gene is part of the presynaptic cytoskeletal matrix, which is involved in establishing active synaptic zones and in synaptic vesicle trafficking. Variations in this gene have been associated with bipolar disorder and major depressive disorder. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

piccolo: May act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 7q21.11

Cellular Component: synaptic vesicle; cytoskeleton; membrane; postsynaptic density; synapse; cell junction

Molecular Function: calcium-dependent phospholipid binding; transporter activity; calcium ion binding; profilin binding

Biological Process: regulation of exocytosis; synaptic vesicle exocytosis; cAMP-mediated signaling; insulin secretion; cytoskeleton organization and biogenesis

Disease: Pontocerebellar Hypoplasia, Type 3