Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prolargin (PRELP) Standard Curve.)
Mouse Prolargin (PRELP) ELISA Kit | PRELP elisa kit
Mouse Prolargin (PRELP) ELISA Kit
Gene Names
Prelp; SLRR2A; 7330409J17Rik
Reactivity
Mouse
Synonyms
Prolargin (PRELP); Mouse Prolargin (PRELP) ELISA Kit; PRELP; MGC45323; MST161; MSTP161; SLRR2A; 55 kDa leucine-rich repeat protein of articular cartilage; prolargin proteoglycan; proline arginine-rich end leucine-rich repeat protein; PRELP elisa kit
Reactivity
Mouse
Specificity
Mouse Prolargin (PRELP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRELP. No significant cross-reactivity or interference between Mouse PRELP and analogues was observed.
Sequence Length
378
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prolargin (PRELP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prolargin (PRELP) Standard Curve.)
Related Product Information for PRELP elisa kit
Description: This Mouse Prolargin (PRELP) ELISA Kit employs a two-site sandwich ELISA to quantitate PRELP in samples. An antibody specific for PRELP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRELP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRELP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRELP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PREPL belongs to the prolyl oligopeptidase subfamily of serine peptidases. Mutations in this gene have been associated with hypotonia-cystinuria syndrome, also known as the 2p21 deletion syndrome. Several alternatively spliced transcript variants encoding either the same or different isoforms have been described for this gene. The longest deduced protein contains 727 amino acids and has a propeller domain in its N-terminal half and a catalytic domain, including the catalytic triad (ser, asp, his) essential for serine peptidases, in its C-terminal half. In sharp contrast to PREP and oligopeptidase B, which require both amino- and carboxy-terminal sequences for activity, PREPL activity appeared to depend only on the carboxy-terminal domain.
Background: PREPL belongs to the prolyl oligopeptidase subfamily of serine peptidases. Mutations in this gene have been associated with hypotonia-cystinuria syndrome, also known as the 2p21 deletion syndrome. Several alternatively spliced transcript variants encoding either the same or different isoforms have been described for this gene. The longest deduced protein contains 727 amino acids and has a propeller domain in its N-terminal half and a catalytic domain, including the catalytic triad (ser, asp, his) essential for serine peptidases, in its C-terminal half. In sharp contrast to PREP and oligopeptidase B, which require both amino- and carboxy-terminal sequences for activity, PREPL activity appeared to depend only on the carboxy-terminal domain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
prolargin
NCBI Official Synonym Full Names
proline arginine-rich end leucine-rich repeat
NCBI Official Symbol
Prelp
NCBI Official Synonym Symbols
SLRR2A; 7330409J17Rik
NCBI Protein Information
prolargin
UniProt Protein Name
Prolargin
Protein Family
UniProt Gene Name
Prelp
UniProt Entry Name
PRELP_MOUSE
Research Articles on PRELP
Similar Products
Product Notes
The Mouse PRELP prelp (Catalog #AAA9717296) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717296 ELISA Kit recognizes Mouse PRELP. It is sometimes possible for the material contained within the vial of "Prolargin (PRELP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
