Mouse anti-Human PC4/SUB1 Monoclonal Antibody | anti-SUB1 antibody

Anti-PC4/SUB1 Antibody, Mouse Monoclonal

Cat. Num. AAA8107312

• Gene Names: SUB1; P15; PC4; p14
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A
• Synonyms: PC4/SUB1; Monoclonal Antibody; Anti-PC4/SUB1 Antibody; Mouse Monoclonal; SUB1/PC4 Antibody; Mouse MAb; SUB1 homolog; transcriptional regulator; Anti-p14 Antibody; Anti-P15 Antibody; Anti-PC4 Antibody; Anti-RPO2TC1 Antibody; Anti-SUB1 Antibody; anti-SUB1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 04
• Specificity: Human SUB1/PC4
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SUB1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ICC/IF: 1:20-1:100; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human SUB1/PC4 protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SUB1/PC4 (rh SUB1/PC4). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(SUB1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-SUB1 mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SUB1 mouse monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa)

Western Blot (WB)

(Anti-SUB1 mouse monoclonal antibody at 1:500 dilutionLane A: NIH-3T3 Whole Cell LysateLane B: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:14 kDaObserved band size:16 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of Human SUB1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human SUB1 monoclonal antibody (1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-mouse IgG secondary antibody(red) and counterstained with DAPI(blue). Positive staining was localized to nucleus.)

Related Product Information for anti-SUB1 antibody

SUB1 belongs to the transcriptional coactivator PC4 family. It is a general coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. SUB1 binds single-stranded DNA. Single-stranded DNA-binding proteins play many roles in nucleic acid metabolism, but their importance during transcription remains unclear. SUB1 exhibits strong genetic interactions with factors necessary for promoter melting. It localizes near the transcription bubble in vitro and binds to promoters in vivo dependent upon preinitiation complexes assembly. SUB1 interacts with the nontemplate strand of RNApII complexes during initiation. It may also be involved in stabilizing the multiprotein transcription complex.

References

Knaus R, et al. (1996) Yeast SUB1 is a suppressor of TFIIB mutations and has homology to the human co-activator PC4. EMBO J. 15(8):1933-40.Ge H, et al. (1994) Purification, cloning, and characterization of a human coactivator, PC4, that mediates transcriptional activation of class II genes. Cell. 78(3):513-23.Kaiser K, et al. (1994) A novel mediator of class II gene transcription with homology to viral immediate-early transcriptional regulators. Cell. 78(3):525-34.

NCBI and Uniprot Product Information

• NCBI GI #: 1709514
• NCBI GeneID: 10923
• UniProt Accession #: P53999; Q96L29
• Molecular Weight: 127
• NCBI Official Full Name: Activated RNA polymerase II transcriptional coactivator p15
• NCBI Official Synonym Full Names: SUB1 homolog (S. cerevisiae)
• NCBI Official Symbol: SUB1
• NCBI Official Synonym Symbols: P15; PC4; p14
• NCBI Protein Information: activated RNA polymerase II transcriptional coactivator p15; positive cofactor 4; activated RNA polymerase II transcription cofactor 4
• UniProt Protein Name: Activated RNA polymerase II transcriptional coactivator p15
• Protein Family: Subtilisin
• UniProt Gene Name: SUB1
• UniProt Synonym Gene Names: PC4; RPO2TC1; PC4
• UniProt Entry Name: TCP4_HUMAN

Uniprot Description

PC4: a transcriptional coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, nonspecifically to double-stranded DNA (ds DNA). Interacts with CSTF2. Its activity is controlled by phosphorylation of the regulatory domain. Phosphorylation inactivates both dsDNA-binding and cofactor function. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.

Protein type: DNA-binding; Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 5p13.3

Cellular Component: transcription factor complex; nucleolus; nucleus

Molecular Function: protein binding; transcription coactivator activity; single-stranded DNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter