Rabbit anti-Human SUB1 Polyclonal Antibody | anti-SUB1 antibody

SUB1, ID (SUB1, PC4, RPO2TC1, Activated RNA polymerase II transcriptional coactivator p15, Positive cofactor 4, SUB1 homolog, p14) (Biotin)

Cat. Num. AAA6352436

• Gene Names: SUB1; P15; PC4; p14
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SUB1; Polyclonal Antibody; ID (SUB1; PC4; RPO2TC1; Activated RNA polymerase II transcriptional coactivator p15; Positive cofactor 4; SUB1 homolog; p14) (Biotin); p14; anti-SUB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-SUB1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SUB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-47 amino acids from the Central region of human SUB1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SUB1 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SUB1 antibody detected the SUB1 protein (arrow).)

Related Product Information for anti-SUB1 antibody

General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).

Product Categories/Family for anti-SUB1 antibody

Antibodies; Enzymes; Polymerase

References

Das, C., et al. J. Mol. Biol. 397(1):1-12(2010). Rajagopalan, S., et al. J. Biol. Chem. 284(32):21728-21737(2009). Mortusewicz, O., et al. J. Cell Biol. 183(5):769-776(2008). Batta, K., et al. Mol. Cell. Biol. 27(21):7603-7614(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 217330646
• NCBI GeneID: 10923
• NCBI Accession #: NP_006704.3
• NCBI GenBank Nucleotide #: NM_006713.3
• UniProt Accession #: P53999; Q96L29
• Molecular Weight: 14,395 Da
• NCBI Official Full Name: activated RNA polymerase II transcriptional coactivator p15
• NCBI Official Synonym Full Names: SUB1 homolog (S. cerevisiae)
• NCBI Official Symbol: SUB1
• NCBI Official Synonym Symbols: P15; PC4; p14
• NCBI Protein Information: activated RNA polymerase II transcriptional coactivator p15; activated RNA polymerase II transcription cofactor 4; positive cofactor 4
• UniProt Protein Name: Activated RNA polymerase II transcriptional coactivator p15
• Protein Family: Subtilisin
• UniProt Gene Name: SUB1
• UniProt Synonym Gene Names: PC4; RPO2TC1; PC4
• UniProt Entry Name: TCP4_HUMAN

Uniprot Description

PC4: a transcriptional coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, nonspecifically to double-stranded DNA (ds DNA). Interacts with CSTF2. Its activity is controlled by phosphorylation of the regulatory domain. Phosphorylation inactivates both dsDNA-binding and cofactor function. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.

Protein type: DNA-binding; Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 5p13.3

Cellular Component: transcription factor complex; nucleolus; nucleus

Molecular Function: protein binding; transcription coactivator activity; single-stranded DNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter