Western Blot (WB)
(Western Blot Positive WB detected in: 293 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, Mouse brain tissue, Rat brain tissue All lanes: PAK2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59 kDa Observed band size: 59 kDa)
PAK2 recombinant antibody
PAK2 Antibody
FC/FACS: 1:20-1:200
IP: 1:200-1:1000
Western Blot (WB)
(Western Blot Positive WB detected in: 293 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, Mouse brain tissue, Rat brain tissue All lanes: PAK2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59 kDa Observed band size: 59 kDa)
Western Blot (WB)
(Western Blot Positive WB detected in: 293 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, Mouse brain tissue, Rat brain tissue All lanes: PAK2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 59 kDa Observed band size: 59 kDa)
Flow Cytometry (FC/FACS)
(Overlay histogram showing HepG2 cells stained with (red line) at 1?50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4?.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4?. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
Flow Cytometry (FC/FACS)
(Overlay histogram showing HepG2 cells stained with (red line) at 1?50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4?.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4?. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
Immunoprecipitation (IP)
(Immunoprecipitating PAK2 in Raji whole cell lysate Lane 1: Rabbit control IgG instead of in Raji whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: Raji whole cell lysate?500ug? Lane 3: Raji whole cell lysate (10ug))
Immunoprecipitation (IP)
(Immunoprecipitating PAK2 in Raji whole cell lysate Lane 1: Rabbit control IgG instead of in Raji whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: Raji whole cell lysate?500ug? Lane 3: Raji whole cell lysate (10ug))
NCBI and Uniprot Product Information
NCBI Description
The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq, Jul 2008]
Uniprot Description
PAK2: a protein kinase of the STE20 family. Critical effector linking RhoGTPases to cytoskeleton reorganization and nuclear signaling. Activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.
Protein type: Protein kinase, STE; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; PAKA subfamily
Chromosomal Location of Human Ortholog: 3q29
Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytosol
Molecular Function: protein serine/threonine kinase activity; identical protein binding; protein binding; protein tyrosine kinase activator activity; protein kinase binding; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity
Biological Process: axon guidance; viral reproduction; apoptosis; protein amino acid autophosphorylation; signal transduction; protein amino acid phosphorylation; T cell receptor signaling pathway; regulation of defense response to virus by virus; regulation of apoptosis; peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; regulation of growth; T cell costimulation; innate immune response; negative regulation of protein kinase activity; mitotic cell cycle; vascular endothelial growth factor receptor signaling pathway; phosphorylation; negative regulation of apoptosis; cell structure disassembly during apoptosis
Research Articles on PAK2
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Product Notes
The PAK2 pak2 (Catalog #AAA7136706) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PAK2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PAK2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:500-1:5000 FC/FACS: 1:20-1:200 IP: 1:200-1:1000. Researchers should empirically determine the suitability of the PAK2 pak2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAK2, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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