Rabbit anti-Mouse PC Polyclonal Antibody | anti-PC antibody

PC, NT (PC, Pyruvate carboxylase, mitochondrial, Pyruvic carboxylase) (AP)

Cat. Num. AAA6331275

• Gene Names: PC; PCB
• Reactivity: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PC; Polyclonal Antibody; NT (PC; Pyruvate carboxylase; mitochondrial; Pyruvic carboxylase) (AP); Pyruvic carboxylase; anti-PC antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PC antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-82 amino acids from the N-terminal region of human PC.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PC Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the PC antibody detected the PC protein (arrow).)

Immunohistochemistry (IHC)

(PC Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PC antibody

This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-PC antibody

Antibodies; Mitochondrial Proteins

References

MacDonald, M.J., et al. Diabetologia 52(6):1087-1091(2009). Monnot, S., et al. Hum. Mutat. 30(5):734-740(2009). Wang, D., et al. Mol. Genet. Metab. 95 (1-2), 31-38 (2008): Vora, S., et al. Natl Med J India 21(3):116-119(2008). Xiang, S., et al. Nat. Struct. Mol. Biol. 15(3):295-302(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 106049528
• NCBI GeneID: 5091
• NCBI Accession #: NP_001035806.1; NP_000911.2
• NCBI GenBank Nucleotide #: NM_001040716.1
• UniProt Accession #: P11498; Q16705; B4DN00
• Molecular Weight: 57,925 Da
• NCBI Official Full Name: pyruvate carboxylase, mitochondrial
• NCBI Official Synonym Full Names: pyruvate carboxylase
• NCBI Official Symbol: PC
• NCBI Official Synonym Symbols: PCB
• NCBI Protein Information: pyruvate carboxylase, mitochondrial
• UniProt Protein Name: Pyruvate carboxylase, mitochondrial
• Protein Family: PCTP-like protein
• UniProt Gene Name: PC
• UniProt Synonym Gene Names: PCB
• UniProt Entry Name: PYC_HUMAN

NCBI Description

This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PC: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate. Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency). PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.

Protein type: Carbohydrate Metabolism - pyruvate; Ligase; Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial; EC 6.4.1.1

Chromosomal Location of Human Ortholog: 11q13.4-q13.5

Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; mitochondrial inner membrane; cytosol

Molecular Function: pyruvate carboxylase activity; protein binding; DNA binding; metal ion binding; biotin carboxylase activity; biotin binding; ATP binding

Biological Process: oxaloacetate metabolic process; vitamin metabolic process; viral RNA genome packaging; carbohydrate metabolic process; glucose metabolic process; pathogenesis; lipid metabolic process; pyruvate metabolic process; water-soluble vitamin metabolic process; biotin metabolic process; gluconeogenesis

Disease: Pyruvate Carboxylase Deficiency