Rabbit anti-Human PAK2 Polyclonal Antibody | anti-PAK2 antibody

PAK2 (Ab-141) Antibody

Cat. Num. AAA9411856

• Gene Names: PAK2; PAK65; PAKgamma
• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: PAK2; Polyclonal Antibody; PAK2 (Ab-141) Antibody; EC 2.7.11.1; gamma-PAK; kinase PAK2; P21-activated kinase 2; p21-activated kinase 2; anti-PAK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total PAK2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 524

• Applicable Applications for anti-PAK2 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:3000
• Immunogen Type: Peptide
• Immunogen Description: Synthesized non-phosphopeptide derived from human PAK2 around the phosphorylation site of serine 141 (Y-L-S(p)-F-T).
• Target Name: PAK2
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, treated with TSA (400nM, 24hours), using PAK2 (Ab-141) antibody.)

Related Product Information for anti-PAK2 antibody

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF-induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation.Martin G.A.,EMBO J. 14:1970-1978(1995).The MGC Project Team;Genome Res. 14:2121-2127(2004).Olsen J.V.,Cell 127:635-648(2006).

Product Categories/Family for anti-PAK2 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 32483399
• NCBI GeneID: 5062
• NCBI Accession #: NP_002568.2
• NCBI GenBank Nucleotide #: NM_002577.4
• UniProt Accession #: Q13177; Q13154; Q6ISC3
• Molecular Weight: 58,043 Da
• NCBI Official Full Name: serine/threonine-protein kinase PAK 2
• NCBI Official Synonym Full Names: p21 protein (Cdc42/Rac)-activated kinase 2
• NCBI Official Symbol: PAK2
• NCBI Official Synonym Symbols: PAK65; PAKgamma
• NCBI Protein Information: serine/threonine-protein kinase PAK 2
• UniProt Protein Name: Serine/threonine-protein kinase PAK 2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PAK2
• UniProt Synonym Gene Names: PAK-2; p27; p34
• UniProt Entry Name: PAK2_HUMAN

NCBI Description

The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq, Jul 2008]

Uniprot Description

PAK2: a protein kinase of the STE20 family. Critical effector linking RhoGTPases to cytoskeleton reorganization and nuclear signaling. Activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; EC 2.7.11.1; STE group; STE20 family; PAKA subfamily

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytosol

Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; protein tyrosine kinase activator activity; protein kinase binding; ATP binding; protein kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: axon guidance; viral reproduction; apoptosis; protein amino acid autophosphorylation; signal transduction; T cell receptor signaling pathway; protein amino acid phosphorylation; regulation of defense response to virus by virus; regulation of apoptosis; positive regulation of peptidyl-tyrosine phosphorylation; peptidyl-serine phosphorylation; regulation of growth; T cell costimulation; negative regulation of protein kinase activity; innate immune response; mitotic cell cycle; vascular endothelial growth factor receptor signaling pathway; phosphorylation; cell structure disassembly during apoptosis; negative regulation of apoptosis