Mouse p97 ATPase Monoclonal Antibody | anti-Vcp antibody

p97 ATPase antibody

Cat. Num. AAA530388

• Gene Names: Vcp; p97; CDC48; p97/VCP; 3110001E05
• Reactivity: Frog, Human, Rabbit, Rat
• Applications: Western Blot
• Purity: Culture supernatant
• Synonyms: p97 ATPase; Monoclonal Antibody; p97 ATPase antibody; Monoclonal p97 ATPase; Anti-p97 ATPase; ATPase p-97; ATPase p97; ATPase p 97; anti-Vcp antibody

• Host: Mouse
• Reactivity: Frog, Human, Rabbit, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 58.13.3
• Specificity: Xenopus laevis p97 ATPase
• Purity/Purification: Culture supernatant
• Form/Format: Concentrated cell culture supernatant with 150mM PBS, 0.1% BSA, and 0.1% NaN3.
• Sequence Length: 5

• Applicable Applications for anti-Vcp antibody: Western Blot (WB)
• Application Notes: WB: 1:1,000-1:2,000
• Biological Significance: p97 ATPase / VCP (also described as valosin-containing protein) is implicated in homotypic membrane fusion events and is related to the N-ethyl maleimide-sensitive fusion protein NSF/Sec18p. The antibody reacts with the p97 subunit of 15S Mg2+-ATPase. p97/VCP is involved in ubiquitin-proteasome dependent protein degradation processes and involvement was also found e.g. in the formation of neuronal inclusion bodies in neuro-degenerative diseseases (e.g. Parkinson?s disease).
• Cross-Reactivity: Frog, human, rabbit, rat
• Biohazard Information: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
• Immunogen: p97 ATPase antibody was raised in mouse using 15S Mg2+ ATPase complex purified from xenopus laevis ovary as the immunogen.
• Preparation and Storage: Store at 4 degree C.

Related Product Information for anti-Vcp antibody

Mouse monoclonal p97 ATPase antibody

Product Categories/Family for anti-Vcp antibody

Proteases; Inhibitors; & Enzymes

References

The Otubain YOD1 Is a Deubiquitinating Enzyme that Associates with p97 to Facilitate Protein Dislocation from the ER; Robert Ernst, Britta Mueller, Hidde L. Ploegh, Christian Schlieker (Molecular Cell: Volume: 36 Issue: 1 Page: 28-38 DOI: 10.1016/j.molcel.2009.09.016)

NCBI and Uniprot Product Information

• NCBI GI #: 4589068
• NCBI GeneID: 269523
• Molecular Weight: 89,322 Da
• NCBI Official Full Name: p97 ATPase, partial
• NCBI Official Synonym Full Names: valosin containing protein
• NCBI Official Symbol: Vcp
• NCBI Official Synonym Symbols: p97; CDC48; p97/VCP; 3110001E05
• NCBI Protein Information: transitional endoplasmic reticulum ATPase
• UniProt Protein Name: Transitional endoplasmic reticulum ATPase
• Protein Family: Vitellogenic carboxypeptidase
• UniProt Gene Name: Vcp
• UniProt Synonym Gene Names: TER ATPase; VCP
• UniProt Entry Name: TERA_MOUSE

Uniprot Description

VCP: valosin-containing protein (VCP) is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the nuclear envelope and of the transitional endoplasmic reticulum (tER). Regulates NFKappaB pathway, which is important for metastasis of osteosarcoma. Tyrosine phosphorylation regulates its cell cycle-dependent nuclear localization.

Protein type: Chaperone; Endoplasmic reticulum; EC 3.6.4.6; Hydrolase; DNA repair, damage

Cellular Component: proteasome complex; nucleoplasm; endoplasmic reticulum membrane; protein complex; intracellular membrane-bound organelle; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; lipid particle; cytosol; nucleus; myelin sheath

Molecular Function: protein domain specific binding; identical protein binding; hydrolase activity; ATPase activity; nucleotide binding; ADP binding; protein phosphatase binding; protein binding; protein complex binding; polyubiquitin binding; lipid binding; ATP binding; receptor binding

Biological Process: ubiquitin-dependent protein catabolic process; caspase activation; ER-associated protein catabolic process; ER to Golgi vesicle-mediated transport; protein ubiquitination; DNA repair; retrograde protein transport, ER to cytosol; bypass DNA synthesis; positive regulation of protein complex assembly; transport; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; double-strand break repair; positive regulation of protein catabolic process; protein amino acid N-linked glycosylation via asparagine; response to DNA damage stimulus; protein homooligomerization